USP24 (NM_015306) Human Recombinant Protein
$2,950.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 294.2 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | In vitro deubiquitination assay (PMID: 27991932) |
| Preparation | NULL or Add: Recombinant proteins was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_056121 |
| Locus ID | 23358 |
| UniProt ID | Q9UPU5 |
| Cytogenetics | 1p32.3 |
| RefSeq ORF | 7860 |
| Summary | Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP24 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 PubMed 14715245).supplied by OMIM, Mar 2008 |
| Protein Categories | Enzyme: Peptidases, Intracellular Proteins |
| Protein Families | Druggable Genome, Protease |
Reviews
Documents
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Resources
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Citations
Deubiquitinase USP24 activated by IL-6/STAT3 enhances PD-1 protein stability and suppresses T cell antitumor response
Hsieh, Young, Chen et al
Sci Adv (2025) 11 (16), eadt4258 DOI: 10.1126/sciadv.adt4258
Hsieh, Young, Chen et al
Sci Adv (2025) 11 (16), eadt4258 DOI: 10.1126/sciadv.adt4258
Cited by 15
AI Summary: OriGene’s human USP24 recombinant protein was used in deubiquitination assays to demonstrate that USP24 directly removes ubiquitin from PD-1, thereby contributing to the study’s findings on PD-1 protein stability and T cell function.
Snippet: …Here, we show that ubiquitin-specific peptidase 24 (USP24) promotes PD-1 protein stability by removing K48-linked polyubiquitin. Increased interleukin-6 level transcriptionally activates the USP24 expression, which leads to PD-1 stabilization. Furthermore, USP24 deficiency reduces PD-1 levels in CD8+ T cells and attenuates EgfrL858R-driven lung tumorigenesis in Usp24C1695A catalytic deficient mice. Targeting PD-1 stability with the USP24-specific inhibitor USP24-i-101 boosts cytotoxic T cell activity, restrains lung tumor growth, and achieves superior therapeutic effects when combined with anti-CTLA4 immunotherapy. Clinically, patients with lung cancer exhibiting high USP24 expression in tumor-infiltrating CD8+ T cells display exhausted features and show unfavorable responses to immunotherapy. Our findings dissect the mechanism for regulating enhanced PD-1 stability in tumor-infiltrating CD8+ T cells and reveal USP24 as a potential target of antitumor immunotherapy;ours simultaneously. … Isolated PD-1 proteins were reacted with or without human USP24 recombinant protein (50 μg/ml) (Origene) in deubiquitination buffer (50 mM tris-HCl, pH 8, 150 mM NaCl, 5 mM MgCl2, and protease inhibitor) for 6 hours at 37°C. …
Defining a Muscle Stem Cell matrisome signature: from transcriptome data to extracellular matrix niche topology
Guillon, Hisoilat, Sasaki et al
bioRxiv (2024)
Guillon, Hisoilat, Sasaki et al
bioRxiv (2024)
Snippet: 1;1/500);Purified polyclonal guinea pig anti-mouse Collagen XIX (see production of Collagen XIX antibodies in the methods section;1/100);rabbit anti-human Aebp1 (Origene TA329346;1/50);rabbit unpurified antisera anti-mouse laminin alpha3AIIIa (1/500);rabbit anti-mouse Laminin (SIGMA L9393;1/400);Chicken anti-GFP (Abcam ab13970;1/1000). …
USP24-i-101 targeting of USP24 activates autophagy to inhibit drug resistance acquired during cancer therapy
Young, Wang, Chen et al
Cell Death Differ (2024)
Young, Wang, Chen et al
Cell Death Differ (2024)
Cited by 29
USP24 promotes drug resistance during cancer therapy
Wang, Young, Wang et al
Cell Death Differ (2021)
Wang, Young, Wang et al
Cell Death Differ (2021)
Cited by 31
AI Summary: OriGene’s human recombinant USP24 protein was used to assess USP24’s deubiquitination activity on P-gp in vitro, helping to elucidate the molecular mechanism by which USP24 promotes drug resistance in cancer therapy.
Snippet: … P-gp protein in A549 cell lysates were immunoprecipitated by incubating with anti-P-gp antibody for 4 h, and then incubated with protein A agarose (Millipore) for 1 h. After washing, substrate was mixed with human recombinant USP24 protein (50 μg per ml) (OriGene) in deubiquitination buffer (50 mM Tris PH 8.0, 10 mM DTT and 5 μM MG132) for 2 h in 37 °C. …
USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy
Wang, Wu, Hung et al
Nat Commun (2018) 9 (1), 3996 DOI: 10.1038/s41467-018-06178-1
Wang, Wu, Hung et al
Nat Commun (2018) 9 (1), 3996 DOI: 10.1038/s41467-018-06178-1
AI Summary: OriGene’s human recombinant USP24 protein was used in deubiquitination assays to investigate the role of USP24 in stabilizing key proteins that promote IL-6 expression and cancer malignancy.
Snippet: …Ubiquitin specific peptidase 24 (USP24) is a 2620 amino acid protein that contains one ubiquitin-associated domain (UBA), which binds to the ubiquitin signal on subs;fter washing, substrate was mixed with human recombinant USP24 protein (50 μg per ml) (Origene) in deubiquitination buffer (50 mM Tris PH 8.0, 10 mM DTT and 5 μM MG132) for 2 h in 37 °C. …
EGF-mediated inhibition of ubiquitin-specific peptidase 24 expression has a crucial role in tumorigenesis
Wang, Wang, Chuang et al
Oncogene (2016)
Wang, Wang, Chuang et al
Oncogene (2016)
AI Summary: OriGene’s recombinant human USP24 protein was used in this study to assess its ability to deubiquitinate cancer-related substrates (Bax, p300, E2F4, and securin), thereby elucidating USP24’s role in regulating cell apoptosis and tumorigenesis.
Snippet: Cell lysates with MG132 (5 μm) treatment were immunoprecipitated with Bax, p300, securin or E2F4 antibody for 4 h, and then incubated with protein A-Sepharose for 1 h. After washing, recombinant human USP24 (Origene, Rockville, MD, USA) was added to the substrates ... Cite SaveSaving
Showing 1-6 of 6 papers.
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