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NDE1 (NM_001143979) Human Recombinant Protein

MVPro

Full-length human proteins expressed in HEK293T cells

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SKU

TP327919L

Recombinant protein of human nudE nuclear distribution gene E homolog 1 (A. nidulans) (NDE1), transcript variant 1, 1 mg

Datasheet SDS

Coomassie blue staining of purified NDE1 protein (Cat# <a href = "https://www.origene.com/catalog/proteins/recombinant-proteins/tp327919-nde1-nm-001143979-human-recombinant-protein" title="TP327919" class="">TP327919</a>). The protein was produced from HEK293T cells transfected with NDE1 cDNA clone (Cat# <a href = "https://www.origene.com/catalog/cdna-clones/expression-plasmids/rc227919-nde1-nm-001143979-human-tagged-orf-clone" title="RC227919" class="">RC227919</a>) using MegaTran 2.0 (Cat# <a href = "https://www.origene.com/catalog/others/transfection-reagents/tt210002-megatran-20-plasmid-dna-transfection-reagent-05-ml" title="TT210002" class="">TT210002</a>).

$9,200.00

6 Weeks*

Specifications

Specifications
Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence >RC227919 protein sequence Red=Cloning site Green=Tags(s) MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNR LRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQ RLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRIPMPSSVEAERTDTAVQATG SVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYD QSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Predicted MW	37.5 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation	Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping	Dry Ice
Reference Data
RefSeq	NP_001137451
Locus ID	54820
UniProt ID	Q9NXR1
Cytogenetics	16p13.11
RefSeq Size	3936
RefSeq ORF	1005
Synonyms	HOM-TES-87; LIS4; MHAC; NDE; NUDE; NUDE1
Summary	This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2012
Protein Categories	Intracellular Proteins

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Documents

FAQs
Protein FAQs
SDS
SDS for TP327919L

Resources

Recombinant Protein Resources
Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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