NF-kB p65 (RELA) (NM_021975) Human Recombinant Protein
SKU
TP320780
Recombinant protein of human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1, 20 µg
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 60 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | RELA Activity Verified in a DNA-binding Assay: RELA activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the RELA consensus DNA-binding sequence was incubated with dilutions of the purified RELA protein. RELA bound to the oligo was captured onto the surface of a microtiter plate and after washing, bound RELA was detected with an anti-RELA primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm. ELISA binding assay (PMID: 25584020) WB positive control (PMID: 25853889) EMSA assay (PMID: 25853889) Binding assay (PMID: 26561547) Pull-down assay (PMID: 26984196) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_068810 |
| Locus ID | 5970 |
| UniProt ID | Q04206 |
| Cytogenetics | 11q13.1 |
| RefSeq Size | 1760 |
| RefSeq ORF | 1653 |
| Synonyms | CMCU; NFKB3; p65 |
| Summary | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2011 |
| Protein Categories | Cytokines, Growth Factors, Immune system diseases, Intracellular Proteins, Transciption Factors |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "NF-kB p65" proteins (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH320780 | RELA MS Standard C13 and N15-labeled recombinant protein (NP_068810) | 10 ug |
$3,360.00
|
|
| LC402895 | Lyophilized RELA HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY402895 | Transient overexpression lysate of v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
| TP750174 | Purified recombinant protein of Human v-rel reticuloendotheliosis viral oncogene homolog A (avian) (p65), transcript variant 1, Ser374-end, with C-terminal Myc-DDK tag, expressed in E.coli, 50ug | 50 ug |
$260.00
|
|
Citations
Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts
Litke, Jaffrey
Nat. Biotechnol. (2019) 37 (6), 667-675 DOI: 10.1038/s41587-019-0090-6
Litke, Jaffrey
Nat. Biotechnol. (2019) 37 (6), 667-675 DOI: 10.1038/s41587-019-0090-6
AI Summary: OriGene’s recombinant p65 protein (No. TP320780) was used in this study to titrate samples for assessing the binding and functional activity of RNA aptamers expressed using the Tornado system.
Snippet: Samples were titrated with recombinant p65 (OriGene Technologies, No. TP320780) from 0.5 to 763 nM. After separation on a 5% polyacrylamide gel containing ×0.25 TBE, RNA was transferred to Hybond-N+ Membranes (GE Healthcare, no. RPN1210B) and subsequently treated with 50 μg mL−1 Proteinase K solution, RNA grade (Thermo Fisher, no. 25530049) in ×1 PBS for 1.5 h
Detecting transcription factors with allosteric DNA-Silver nanocluster switches
Li, Chen, Wang et al
Analytica Chimica Acta (2018)
Li, Chen, Wang et al
Analytica Chimica Acta (2018)
AI Summary: OriGene products, specifically the microphthalmia-associated transcription factor (MITF) and p65 proteins, were used as target transcription factors to validate the detection capability of the allosteric DNA-silver nanocluster switches described in the study.
Snippet: TATA box binding protein (TBP, ∼39 kDa) was obtained from ActiveMotif (CA, USA). Microphthalmia-associated transcription factor (MITF, ∼46.8 kDa) and p65 (∼60kDa) were purchased from OriGene Technologies (MD, USA)
Novel Nuclear Factor-KappaB Targeting Peptide Suppresses β-Amyloid Induced Inflammatory and Apoptotic Responses in Neuronal Cells
Srinivasan, Bayon, Chopra et al
PLoS One (2016) 11 (10), e0160314 DOI: 10.1371/journal.pone.0160314
Srinivasan, Bayon, Chopra et al
PLoS One (2016) 11 (10), e0160314 DOI: 10.1371/journal.pone.0160314
Cited by 18
AI Summary: In this study, OriGene’s anti-NFκB p65 antibody (Cat# TA890015) was used to detect and quantify NF-κB p65 protein levels, enabling assessment of the novel peptide’s impact on NF-κB signaling in neuronal cells.
FOXO3-NF-κB RelA Protein Complexes Reduce Proinflammatory Cell Signaling and Function
Thompson, Larson, Vidrine et al
J. Immunol. (2015)
Thompson, Larson, Vidrine et al
J. Immunol. (2015)
Cited by 54
MYB Elongation Is Regulated by the Nucleic Acid Binding of NFκB p50 to the Intronic Stem-Loop Region
Pereira, Hugo, Malaterre et al
PLoS ONE (2015) 10 (4), e0122919 DOI: 10.1371/journal.pone.0122919
Pereira, Hugo, Malaterre et al
PLoS ONE (2015) 10 (4), e0122919 DOI: 10.1371/journal.pone.0122919
Cited by 21
AI Summary: OriGene contributed to this study by providing the NFκBp65 protein, which was used in DNA binding assays to investigate the regulation of MYB transcriptional elongation.
Snippet: NFκBp65 DNA EMSAs were performed with 32 P-labeled HIV-1 LTR DNA probe [33] and NFκBp65 protein (Origene) in reactions containing 25 mM HEPES-KOH pH7.9, 0.5mM EDTA, 50 mM NaCl, 5% glycerol, and 1% NP40. ... NFκBp65 was obtained from Origene, USA ... Cite SaveSaving
Functional characterization of a competitive peptide antagonist of p65 in human macrophage-like cells suggests therapeutic potential for chronic inflammation
Srinivasan, Blackburn, Lahiri
Drug Des Devel Ther (2014) 8, 2409-21 DOI: 10.2147/DDDT.S59722
Srinivasan, Blackburn, Lahiri
Drug Des Devel Ther (2014) 8, 2409-21 DOI: 10.2147/DDDT.S59722
AI Summary: In this study, OriGene's anti-NFκB p65 antibody (TA328076) was used to assess nuclear translocation of p65 in human macrophage-like THP-1 cells, helping to demonstrate the efficacy of the GILZ-derived peptide antagonist in suppressing inflammatory responses.
Showing 1-6 of 8 papers.
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