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SHP2 (PTPN11) (NM_002834) Human Recombinant Protein

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SKU
TP320029
Recombinant protein of human protein tyrosine phosphatase, non-receptor type 11 (PTPN11), 20 µg
Size:
20 ug 1 mg 100 ug
  $737.00
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Frequently Bought Together (2)

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC220029 representing NM_002834
Red=Cloning site Green=Tags(s)

MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEK
FATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRES
QSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVL
QLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYK
NILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENS
RVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHF
RTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDV
PKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPL
PPCTPTPPCAEMREDSARVYENVGLMQQQKSFR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 67.8 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_002825
Locus ID 5781
UniProt ID Q06124
Cytogenetics 12q24.13
RefSeq Size 6300
RefSeq ORF 1779
Synonyms BPTP3; CFC; JMML; METCDS; NS1; PTP-1D; PTP2C; SH-PTP2; SH-PTP3; SHP2
Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. provided by RefSeq, Aug 2016
Protein Categories Cancer: Haematopoietic and Lymphoid, Cytokines, Growth Factors, Intracellular Proteins
Protein Families Druggable Genome, Phosphatase
Protein Pathways Adipocytokine signaling pathway, Chronic myeloid leukemia, Epithelial cell signaling in Helicobacter pylori infection, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Renal cell carcinoma
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Other "SHP2" proteins (6)
SKU Description Size Price
PH320029 PTPN11 MS Standard C13 and N15-labeled recombinant protein (NP_002825) 10 ug
$3,360.00
TP750155 Purified recombinant protein of PTPN11, Met1-Leu525,expressed in E. coli,50ug 50 ug
$270.00
TP750156 Purified recombinant protein of PTPN11, Met1-Leu525(mutant Glu76Lys),expressed in E. coli,50ug. 50 ug
$270.00
TP750157 Purified recombinant protein of PTPN11, Met1-Leu525(mutant Asp61Tyr),expressed in E. coli,50ug 50 ug
$270.00
TP750158 Purified recombinant protein of PTPN11, Met1-Leu525(mutant Ser502Leu),expressed in E. coli,50ug 50 ug
$270.00
TP750164 Purified recombinant protein of Human protein tyrosine phosphatase, non-receptor type 11 (PTPN11), Ala237-Ile529, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

Inhibition of Phosphatase Activity Follows Decline in Sulfatase Activity and Leads to Transcriptional Effects through Sustained Phosphorylation of Transcription Factor MITF
Bhattacharyya, Feferman, Tobacman
PLoS ONE (2016) 11 (4), e0153463 DOI: 10.1371/journal.pone.0153463
Cited by 31
AI Summary: OriGene’s ARSB (NM_000046) plasmid in the pCMV6-XL4 vector was used to overexpress ARSB in HepG2 cells, enabling the study of how restoring ARSB levels affects downstream signaling and transcriptional regulation in the context of phosphatase and sulfatase activity.
Snippet: Transfections of ARSB and SHP2. ARSB (NM_000046) plasmid in pCMV6-XL4 vector was obtained (Origene), and overexpressed in HepG2 cells by transient transfection using 2 μg of the plasmid and Lipofectamine 2000 (Invitrogen) [20,22]. ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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