Nucleolin (NCL) (NM_005381) Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 76.4 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | EMSA reaction positive control (PMID: 26354862) Taq polymerase assay (regulator) (PMID: 26354862) Binding assay (Dimethylsulfate footprinting) (PMID: 26354862) Binding assay (FRET) (PMID: 26354862) Surface Plasmon Ressonance (SPR) (PMID: 26354862) Association in cell culture (PMID: 26707270) Surface Plasmon Ressonance (SPR) (PMID: 27032748) EMSA reaction positive control (PMID: 27913192) ELISA binding assay (PMID: 28974366) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_005372 |
| Locus ID | 4691 |
| UniProt ID | P19338 |
| Cytogenetics | 2q37.1 |
| RefSeq Size | 2732 |
| RefSeq ORF | 2130 |
| Synonyms | C23; Nsr1 |
| Summary | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. provided by RefSeq, Jul 2008 |
| Protein Categories | Growth Factors, Intracellular Proteins |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Pathogenic Escherichia coli infection |
Reviews
Documents
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Resources
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Citations
Soluble TREM2 engages cell-surface nucleolin to drive vascular permeability and malignant ascites in ovarian cancer
Li, Zhang, Luo et al
EMBO Mol Med (2026)
Li, Zhang, Luo et al
EMBO Mol Med (2026)
Cited by 1
AI Summary: OriGene’s Human NCL recombinant protein (TP319082) was used in this study to investigate the interaction between soluble TREM2 and cell-surface nucleolin, helping to elucidate the mechanism by which sTREM2 promotes vascular permeability and malignant ascites in ovarian cancer.
Snippet: Biotinylated human sTREM2-His Sino Biological, China 11084-H49H-B Human NCL recombinant protein OriGene, USA TP319082
Design and evaluation of dual-functional aptamer-peptide conjugates as a platform for targeted cancer therapy
Marzano, Ferraro, Grasso et al
Eur J Pharm Sci (2026) 218, 107428 DOI: 10.1016/j.ejps.2026.107428
Marzano, Ferraro, Grasso et al
Eur J Pharm Sci (2026) 218, 107428 DOI: 10.1016/j.ejps.2026.107428
Cited by 2
AI Summary: OriGene’s recombinant nucleolin protein (NCL, TP319082) was used as the immobilized target in surface plasmon resonance experiments to assess the binding affinity of the designed aptamer-peptide conjugates in this study.
Snippet: AS1411 is a G-rich DNA aptamer that exhibits intrinsic antitumor activity through selective binding to nucleolin, a protein overexpressed in many cancers. Beyond its;Surface Plasmon Resonance (SPR) experiments were performed at 25 °C on a Biacore X100 (GE Healthcare). The nucleolin protein (NCL, TP319082, OriGene Technologies GmbH, Germany) was immobilized on a research-grade CM5 sensor chip via standard
Development of RNA G-quadruplex (rG4)-targeting L-RNA aptamers by rG4-SELEX
Umar, Chan, Kwok
Nat Protoc (2022)
Umar, Chan, Kwok
Nat Protoc (2022)
Cited by 31
AI Summary: In this study, OriGene's Nucleolin protein (cat. no. TP319082) was used as a target protein to evaluate the binding specificity and inhibitory potential of the developed L-RNA aptamers against rG4-protein interactions.
Snippet: RNA G-quadruplex (rG4)-SELEX is a method that generates l-RNA aptamers to target an rG4 structure of interest, which can be applied to inhibit G-quadruplex-mediated Save;RHAU53 peptide (Synpeptide) Nucleolin protein (OriGene, cat. no. TP319082) Q5 Hot Start High-Fidelity Master Mix (NEB, cat. no. …no. NC0281066)
Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis
Kirman, Renganathan, Chui et al
Cell Death Dis (2022) 13 (2), 172 DOI: 10.1038/s41419-022-04618-x
Kirman, Renganathan, Chui et al
Cell Death Dis (2022) 13 (2), 172 DOI: 10.1038/s41419-022-04618-x
Cited by 21
AI Summary: In this study, OriGene products, specifically human nucleolin shRNA (OriGene, #TG311273) and human nucleolin cDNA (OriGene, #SC119469), were used to manipulate nucleolin expression levels in endothelial cells, enabling the authors to elucidate the role of cell surface nucleolin as a receptor mediating ADAMTS5-induced endothelial cell apoptosis.
Snippet: ases [11] and embryonic development [12–14]). We previously reported a novel role of ADAMTS5 as an angiogenesis inhibitor, suppressing tumor growth [15, 16]. In the subcutaneous xenograft mouse tumorigenesis model, B16 melanoma cells overexpressing full-length ADAMTS5 and TS5-p45 truncate both suppressed tumor angiogenesis and enhanced apoptosis within the tumor, resulting in tumor growth suppr
Toward G-Quadruplex-Based Anticancer Agents: Biophysical and Biological Studies of Novel AS1411 Derivatives
Ogloblina, Iaccarino, Capasso et al
Int J Mol Sci (2020) 21 (20) DOI: 10.3390/ijms21207781
Ogloblina, Iaccarino, Capasso et al
Int J Mol Sci (2020) 21 (20) DOI: 10.3390/ijms21207781
Cited by 26
AI Summary: OriGene's Human Recombinant Nucleolin Protein (NM_005381) was used in this study to assess the binding affinity of novel AS1411 derivatives to nucleolin, contributing to the evaluation of their anticancer potential.
Snippet: The Human Recombinant Nucleolin Protein (NM_005381) was purchased from OriGene Technologies GmbH (Herford, Germany). Phosphoramidite and controlled pore glass supports for DNA synthesis were purchased from Link Technologies (Bellshill, UK)
Specific suppression of D-RNA G-quadruplex-protein interaction with an L-RNA aptamer
Umar, Kwok
Nucleic Acids Res. (2020) 48 (18), 10125-10141 DOI: 10.1093/nar/gkaa759
Umar, Kwok
Nucleic Acids Res. (2020) 48 (18), 10125-10141 DOI: 10.1093/nar/gkaa759
Cited by 55
AI Summary: OriGene's nucleolin protein and RHAU53 peptide were used in this study to assess the binding and inhibition effects of the novel L-RNA aptamer on rG4-protein interactions.
Snippet: ans5α chemically competent cells used for cloning were purchased from Transgen Biotech (Beijing). The RHAU53 peptide and nucleolin protein used for the binding and inhibition study were ordered from OriGene. With the exception of last mining cycle, all PCRs amplifications were carried out with NEB’s Q5 Hot Start High-Fidelity Master Mix, while Thermo Scientific's DreamTaq Polymerase was used in;- ...
Showing 1-6 of 14 papers.
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