MDA5 (IFIH1) (NM_022168) Human Recombinant Protein
SKU
TP315661M
Recombinant protein of human interferon induced with helicase C domain 1 (IFIH1), 100 µg
$2,950.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 116.5 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | ELISA capture for autoantibodies (PMID: 28487565) ELISA capture for autoantibodies (PMID: 28842784) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_071451 |
| Locus ID | 64135 |
| UniProt ID | Q9BYX4 |
| Cytogenetics | 2q24.2 |
| RefSeq Size | 3434 |
| RefSeq ORF | 3075 |
| Synonyms | AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2; SGMRT1 |
| Summary | IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndromeprovided by RefSeq, Jul 2020 |
| Protein Categories | Cytokines, Diabetes, Immune system diseases, Intracellular Proteins |
| Protein Pathways | RIG-I-like receptor signaling pathway |
Reviews
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Resources
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Citations
A non-radioactive modified immunoprecipitation assay for diagnostic detection of anti-MDA5 antibodies in idiopathic inflammatory myopathies
Wang, Teo, Lan et al
Front Immunol (2026) 17, 1896104-1896104 DOI: 10.3389/fimmu.2026.1896104
Wang, Teo, Lan et al
Front Immunol (2026) 17, 1896104-1896104 DOI: 10.3389/fimmu.2026.1896104
AI Summary: In this study, OriGene's recombinant human MDA5 protein (cat. no. TP315661) was used as a positive protein control to validate the modified immunoprecipitation assay for detecting anti-MDA5 antibodies in idiopathic inflammatory myopathies.
Snippet: g at approximately 95 °C for 10 min.Immunoprecipitated proteins were separated by Bis-Tris SDS-PAGE and transferred onto polyvinylidene difluoride membranes. Recombinant human MDA5 protein (OriGene Technologies;cat. no. TP315661) was used as a positive protein control. Immunoprecipitated MDA5 was detected using a rabbit monoclonal anti-MDA5 antibody (clone D74E4;Cell Signaling Technolo
Increased Serum Matrix Metalloproteinase-9 Levels are Associated with Anti-Jo1 but not Anti-MDA5 in Myositis Patients
Liu, Luo, Wang et al
Aging Dis (2019) 10 (4), 746-755 DOI: 10.14336/AD.2018.1120
Liu, Luo, Wang et al
Aging Dis (2019) 10 (4), 746-755 DOI: 10.14336/AD.2018.1120
AI Summary: OriGene’s recombinant full-length MDA5 antigen was used to coat ELISA plates for the detection and quantification of anti-MDA5 antibodies in patient serum, enabling the study’s analysis of serological differences in myositis patients.
Snippet: Briefly, 96-well microplates were coated with 0.5 µg/ml recombinant full-length MDA5 antigen (Origene Technologies, MD, USA) overnight at 4°C. …The antibody units were calculated from the optimal densities at 450 nm using a standard curve obtained from serial concentrations of serum containing anti-MDA5 antibodies
Neutrophil extracellular traps may contribute to interstitial lung disease associated with anti-MDA5 autoantibody positive dermatomyositis
Peng, Zhang, Zhao et al
Clin. Rheumatol. (2017)
Peng, Zhang, Zhao et al
Clin. Rheumatol. (2017)
AI Summary: OriGene’s purified recombinant MDA5 protein was used in ELISA assays to detect anti-MDA5 autoantibody levels, enabling the study to assess their association with interstitial lung disease in dermatomyositis patients.
Snippet: … Detection of anti-MDA5 autoantibody and KL-6 Anti-MDA5 autoAb titers were determined by ELISA using purified recombinant protein of MDA5 (OriGene, Maryland, USA) as described previously [6, 17]. ...;... … Detection of anti-MDA5 autoantibody and KL-6 Anti-MDA5 autoAb titers were determined by ELISA using purified recombinant protein of MDA5 (OriGene, Maryland, USA) as described previously [6, 17]. ... …
Chemokine profiles of interstitial pneumonia in patients with dermatomyositis: a case control study
Oda, Kotani, Takeuchi et al
Sci Rep (2017) 7 (1), 1635 DOI: 10.1038/s41598-017-01685-5
Oda, Kotani, Takeuchi et al
Sci Rep (2017) 7 (1), 1635 DOI: 10.1038/s41598-017-01685-5
AI Summary: OriGene's recombinant MDA5 antigen was used in ELISA assays to detect anti-MDA5 antibodies in patient serum, contributing to the evaluation of autoantibody profiles in the study.
Snippet: 125.3, 3.54, and 2.19 pg/ml, respectively. Anti-MDA5 antibody was determined by ELISA using recombinant MDA5 antigen (OriGene, Rockville, MD, USA) as described previously 36 . Anti-ARS antibody was determined using ... All 3 versions Cite SaveSaving
Increased Serum LIGHT Levels Correlate with Disease Progression and Severity of Interstitial Pneumonia in Patients with Dermatomyositis: A Case Control Study
Kotani, Takeuchi, Ishida et al
PLoS ONE (2015) 10 (10), e0140117 DOI: 10.1371/journal.pone.0140117
Kotani, Takeuchi, Ishida et al
PLoS ONE (2015) 10 (10), e0140117 DOI: 10.1371/journal.pone.0140117
Cited by 10
AI Summary: OriGene's recombinant MDA5 antigen was used in ELISA assays to measure anti-MDA5 antibody levels in patient serum, aiding in the clinical assessment of dermatomyositis with interstitial pneumonia.
Snippet: ml, respectively. Anti-melanoma differentiation-associated gene 5 (MDA5) antibody (Ab) was determined by ELISA using recombinant MDA5 antigen (OriGene, Rockville, MD, USA) as described previously [26]. Anti-aminoacyl ... Cite SaveSaving
Anti-MDA5 autoantibodies in juvenile dermatomyositis identify a distinct clinical phenotype: a prospective cohort study
Tansley, Betteridge, Gunawardena et al
Arthritis Res. Ther. (2014) 16 (4), R138 DOI: 10.1186/ar4600
Tansley, Betteridge, Gunawardena et al
Arthritis Res. Ther. (2014) 16 (4), R138 DOI: 10.1186/ar4600
Cited by 195
AI Summary: OriGene’s recombinant MDA5 protein (rMDA-5) was used to coat ELISA plates, enabling the detection of anti-MDA5 autoantibodies in juvenile dermatomyositis patient sera and thereby helping to identify a distinct clinical phenotype associated with these antibodies.
Snippet: Labelled proteins were analysed by autoradiography. ELISA ELISA assays were performed as previously described [2] with some modifications. 96-well polystyrene plates were coated with rMDA-5 (Origene) at 4°C for 16 hours. Serum samples were diluted to 1:250 ... Cite SaveSaving
Showing 1-6 of 9 papers.
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