MEKK1 (MAP3K1) (NM_005921) Human Recombinant Protein
SKU
TP314358
Purified recombinant protein of Homo sapiens mitogen-activated protein kinase kinase kinase 1 (MAP3K1), 20 µg
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 164.3 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_005912 |
| Locus ID | 4214 |
| UniProt ID | Q13233 |
| Cytogenetics | 5q11.2 |
| RefSeq Size | 7522 |
| RefSeq ORF | 4536 |
| Synonyms | MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6 |
| Summary | The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. provided by RefSeq, Mar 2012 |
| Protein Categories | Enzyme: Kinases, Membrane Proteins |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, RIG-I-like receptor signaling pathway, Ubiquitin mediated proteolysis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "MEKK1" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH314358 | MAP3K1 MS Standard C13 and N15-labeled recombinant protein (NP_005912) | 10 ug |
$3,360.00
|
|
Citations
Combating acquired resistance to MAPK inhibitors in melanoma by targeting Abl1/2-mediated reactivation of MEK/ERK/MYC signaling
Tripathi, Liu, Jain et al
Nat Commun (2020) 11 (1), 5463 DOI: 10.1038/s41467-020-19075-3
Tripathi, Liu, Jain et al
Nat Commun (2020) 11 (1), 5463 DOI: 10.1038/s41467-020-19075-3
Cited by 51
AI Summary: OriGene’s recombinant MAP3K1 protein was used in kinase assays to investigate ABL1/2-mediated phosphorylation, helping to elucidate the molecular mechanisms underlying resistance to MAPK inhibitors in melanoma.
Snippet: … Incubation of MAP3K1 with ABL1 or ABL2 results in modest tyrosine phosphorylation of full-length MAP3K1 (Fig. 7c). The supplied recombinant MAP3K1 has some degradation products (see Origene website). Interestingly, ABL1 efficiently phosphorylates the 80 kDa breakdown product, whereas ABL1 and ABL2 phosphorylate a 100 kDa breakdown product recognized by a C-terminal MAP3K1 ant;. The supplied recombinant MAP3K1 has some degradation products (see Origene website). Interestingly, ABL1 efficiently phosphorylates the 80 kDa breakdown product, whereas ABL1 and ABL2 phosphorylate a 100 kDa breakdown product recognized by a C-terminal MAP3K1 antibody (Fig. 7d, left and Supplementary Fig. 7a, left)
Showing 1-1 of 1 papers.
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