NLN (NM_020726) Human Recombinant Protein

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SKU
TP312447
Recombinant protein of human neurolysin (metallopeptidase M3 family) (NLN), 20 µg
  $867.00
4 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC212447 representing NM_020726
Red=Cloning site Green=Tags(s)

MIARCLLAVRSLRRVGGSRILLRMTLGREVMSPLQAMSSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQ
VYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRG
DIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDD
TFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIIL
QQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGF
EYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTL
YTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHD
EVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLL
EKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYD
GQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRG
LHAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 80.5 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_065777
Locus ID 57486
UniProt ID Q9BYT8
Cytogenetics 5q12.3
RefSeq Size 2882
RefSeq ORF 2112
Synonyms AGTBP; EP24.16; MEP; MOP
Summary This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.provided by RefSeq, Jun 2010
Protein Categories Enzyme: Peptidases, Intracellular Proteins
Protein Families Druggable Genome, Protease
Protein Pathways Renin-angiotensin system
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Documents
Resources
Other "NLN" proteins (3)
SKU Description Size Price
PH312447 NLN MS Standard C13 and N15-labeled recombinant protein (NP_065777) 10 ug
$3,360.00
LC412353 Lyophilized NLN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY412353 Transient overexpression lysate of neurolysin (metallopeptidase M3 family) (NLN) (as WB positive control) 100 ug
$665.00

file-alt Citations

Analysis of the proteolysis of bioactive peptides using a peptidomics approach
Kim, Lone, Saghatelian
Nat Protoc (2013) 8 (9), 1730-42 DOI: 10.1038/nprot.2013.104
Cited by 28
AI Summary: OriGene products, specifically the insulin-degrading enzyme (IDE) cDNA (Cat# SC119387) and IDE shRNA plasmids (Cat# TL506959), were used in this study to overexpress or knock down IDE in cells, enabling the authors to investigate and validate the role of IDE in the proteolysis of bioactive peptides.
Snippet: that this peptide may be a valuable therapeutic. Unfortunately, GLP-1 has a short half-life because of proteolysis 12, 23 , which prevents it from being an effective drug. Consequently, the focus shifted toward the identification
Showing 1-1 of 1 papers.
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