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SHFL (NM_018381) Human Recombinant Protein

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SKU
TP312344L
Recombinant protein of human chromosome 19 open reading frame 66 (C19orf66), 1 mg
Size:
1 mg 20 ug 100 ug
  $9,200.00
6 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC212344 representing NM_018381
Red=Cloning site Green=Tags(s)

MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPED
MKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYE
PVPADKMWGLAEFHCPKCRHNFRGWAQMGSPSPCYGCGFPVYPTRILPPRWDRDPDRRSTHTHSCSAADC
YNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEE
EVEDEEGGPRE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 32.9 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_060851
Locus ID 55337
UniProt ID Q9NUL5
Cytogenetics 19p13.2
RefSeq Size 1911
RefSeq ORF 873
Synonyms C19orf66; IRAV; RyDEN; SFL
Summary Exhibits antiviral activity against dengue virus (DENV) and can inhibit the replication of all DENV serotypes. May block the protein translation of DENV RNA via its association with cellular mRNA-binding proteins and viral RNA. Can also limit the replication of hepatitis C virus (HCV), West Nile virus (WNV), Chikungunya virus (CHIKV), herpes simplex virus type 1 (HHV-1) and human adenovirus (PubMed:26735137).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
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Documents
Resources

file-alt Citations

EGR1 functions as a new host restriction factor for SARS-CoV-2 to inhibit virus replication through the E3 ubiquitin ligase MARCH8
Zhao, Sui, Wu et al
J Virol (2023)
Cited by 23
AI Summary: OriGene products—recombinant IRAV (TP312344) and MARCH8 (TP311771) proteins—were used in this study to assess their binding affinity with the SARS-CoV-2 N protein, helping to elucidate the mechanism by which EGR1 inhibits viral replication.
Snippet: The affinity of the SARS-CoV-2 N protein (catalog no. PCN001;CRGEN Technologies) with the recombinant proteins IRAV and MARCH8 (catalog no. TP312344 and TP311771;OriGene Technologies) was calculated using Monolith NT
Showing 1-1 of 1 papers.
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