TBC1D4 (NM_014832) Human Recombinant Protein
$7,820.00
6 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 146.4 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_055647 |
| Locus ID | 9882 |
| UniProt ID | O60343 |
| Cytogenetics | 13q22.2 |
| RefSeq Size | 5922 |
| RefSeq ORF | 3897 |
| Synonyms | AS160; NIDDM5 |
| Summary | This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Aug 2015 |
| Protein Categories | Intracellular Proteins |
Reviews
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Resources
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Citations
WNK1 phosphorylation sites in TBC1D1 and TBC1D4 modulate cell surface expression of GLUT1
Henriques, Matos, Carvalho et al
Arch. Biochem. Biophys. (2019) 679, 108223 DOI: 10.1016/j.abb.2019.108223
Henriques, Matos, Carvalho et al
Arch. Biochem. Biophys. (2019) 679, 108223 DOI: 10.1016/j.abb.2019.108223
Cited by 29
AI Summary: In this study, recombinant rMyc-TBC1D1 and rMyc-TBC1D4 proteins from OriGene were used as substrates in kinase assays to investigate how WNK1 phosphorylation affects GLUT1 cell surface expression.
Snippet: were incubated in 20 μL kinase reaction buffer (30 mM Tris-HCl pH 7.5, 10% glycerol, 1 mM DTT, 1 mM Na 3 VO 4 , 10 mM MgCl 2 , 100 μM ATP), mixed with their recombinant substrate (0.5 μg of rMyc-TBC1D1 or rMyc-TBC1D4 (both from Origene Technologies, Rockville, USA;- were incubated in 20 μL kinase reaction buffer (30 mM Tris-HCl pH 7.5, 10% glycerol, 1 mM DTT, 1 mM Na 3 VO 4 , 10 mM MgCl 2 , 100 μM ATP), mixed with their recombinant substrate (0.5 μg of rMyc-TBC1D1 or rMyc-TBC1D4 (both from Origene Technologies, Rockville
Showing 1-1 of 1 papers.
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