TDP43 (TARDBP) (NM_007375) Human Recombinant Protein
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 44.6 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | In vitro binding assay (PMID: 25738460) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_031401 |
| Locus ID | 23435 |
| UniProt ID | Q13148 |
| Cytogenetics | 1p36.22 |
| RefSeq Size | 4236 |
| RefSeq ORF | 1242 |
| Synonyms | ALS10; TDP-43 |
| Summary | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. provided by RefSeq, Jul 2008 |
| Protein Categories | Intracellular Proteins, Nervous system: Neurodegenerative diseases |
| Protein Families | Transcription Factors |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "TDP43" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH310639 | TARDBP MS Standard C13 and N15-labeled recombinant protein (NP_031401) | 10 ug |
$3,360.00
|
|
| LC402137 | Lyophilized TARDBP HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY402137 | Transient overexpression lysate of TAR DNA binding protein (TARDBP) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
TDP-43 stabilizes G3BP1 mRNA: relevance to amyotrophic lateral sclerosis/frontotemporal dementia
Sidibé, Khalfallah, Xiao et al
Brain (2021)
Sidibé, Khalfallah, Xiao et al
Brain (2021)
Cited by 67
AI Summary: OriGene’s recombinant TDP-43 protein (NM_007375) was used in binding assays to investigate the direct interaction between TDP-43 and G3BP1 mRNA, helping to elucidate TDP-43’s role in stabilizing G3BP1 transcripts relevant to ALS/FTD pathology.
Snippet: prepared according to the manufacturer's instructions. 100 µg of lysates or 100 ng of TDP-43 recombinant protein (NM_007375, OriGene) was incubated with 30 pmol of biotin-labelled RNA for 1 h at 4°C. Proteins were eluted with 2.5× Laemmli buffer and immediately
A Liver-Enriched Long Non-Coding RNA, lncLSTR, Regulates Systemic Lipid Metabolism in Mice
Li, Ruan, Yang et al
Cell Metab. (2015) 21 (3), 455-67 DOI: 10.1016/j.cmet.2015.02.004
Li, Ruan, Yang et al
Cell Metab. (2015) 21 (3), 455-67 DOI: 10.1016/j.cmet.2015.02.004
Cited by 284
AI Summary: OriGene’s recombinant TDP-43 protein (catalog #TP310639) was used in binding assays to investigate the interaction between TDP-43 and the Cyp8b1 promoter, helping elucidate the molecular mechanisms by which lncLSTR regulates lipid metabolism in mice.
Snippet: Recombinant Protein Binding Assay 400 ng biotin-labeled Cyp8b1 promoter fragments and 1 ug recombinant TDP-43 protein (OriGene, TP310639) were incubated in 300 ul binding buffer (0.1 mg/ml BSA, 10 ng/ul sperm DNA (Sigma, D7656), 5% glycerol, 0.5 mM DTT, 20 mM HEPES,50 mM KCl,1 mM MgCl2, 0.2% NP-40) for 30 min at 25C. 30 ul streptavidin beads were added into the binding reaction and incubated for another 30 min. …
Full-length TDP-43 forms toxic amyloid oligomers that are present in frontotemporal lobar dementia-TDP patients
Fang, Tsai, Chang et al
Nat Commun (2014) 5, 4824 DOI: 10.1038/ncomms5824
Fang, Tsai, Chang et al
Nat Commun (2014) 5, 4824 DOI: 10.1038/ncomms5824
Cited by 211
AI Summary: OriGene Technologies provided the recombinant full-length human TDP-43 protein (TrueORF clone RC210639 with a C-terminal Myc-DDK tag), which was essential for studying the structural and toxic properties of TDP-43 oligomers in this research.
Snippet: … (Rockville, MD, USA). Based on the product description, TDP-43 was obtained from transiently transfected human HEK 293 cells with a TrueORF clone, RC210639. …
Dual vulnerability of TDP-43 to calpain and caspase-3 proteolysis after neurotoxic conditions and traumatic brain injury
Yang, Lin, Robertson et al
J. Cereb. Blood Flow Metab. (2014)
Yang, Lin, Robertson et al
J. Cereb. Blood Flow Metab. (2014)
Cited by 76
AI Summary: OriGene’s purified recombinant human TDP-43 protein was used in in vitro protease digestion experiments to map TDP-43 fragmentation patterns, helping elucidate its vulnerability to calpain and caspase-3 after neurotoxic conditions and traumatic brain injury.
Snippet: In vitro digestion of cortex lysate (100 μg) or purified recombinant human TDP-43 protein (3 μg, OriGene, MD, USA) was performed with human erythrocyte calpain-1 (Calbiochem/EMD Bioscience, Billerica, MA, USA) and human recombinant caspase-3 (BD ... Cite SaveSaving
Showing 1-4 of 4 papers.
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