eNOS (NOS3) (NM_000603) Human Recombinant Protein
SKU
TP309228M
Recombinant protein of human nitric oxide synthase 3 (endothelial cell) (NOS3), 100 µg
$2,508.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 133.1 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_000594 |
| Locus ID | 4846 |
| UniProt ID | P29474 |
| Cytogenetics | 7q36.1 |
| RefSeq Size | 4345 |
| RefSeq ORF | 3609 |
| Synonyms | ECNOS; eNOS |
| Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. provided by RefSeq, Oct 2016 |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Endothelial Nitric Oxide Synthase-Dependent Mechanism of Hydroxyurea-Induced S-Phase Arrest in Erythroid Cells
Dragojević, Đikić, Mojsilović et al
Antioxidants (2026) 15 (4), 435-435 DOI: 10.3390/antiox15040435
Dragojević, Đikić, Mojsilović et al
Antioxidants (2026) 15 (4), 435-435 DOI: 10.3390/antiox15040435
Cited by 1
AI Summary: OriGene’s human recombinant NOS3 protein (catalog number TP309228M) was used in this study to investigate the biochemical and functional role of NOS3 in hydroxyurea-induced S-phase arrest in erythroid cells.
Snippet: The reaction mixture consisted of 25 mM Tris-HCl, pH 7.4, 3 μM tetrahydrobiopterin, 1 μM flavin adenine dinucleotide, 1 μM flavin adenine mononucleotide, 2.5 mM L-arginine, 1.25 mM nicotinamide adenine dinucleotide phosphate, 0.75 mM calcium chloride, and 1 µM calmodulin. Human recombinant NOS3 protein (OriGene, TP309228M, Rockville
Interaction of cepharanthine with immobilized heat shock protein 90α (Hsp90α) and screening of Hsp90α inhibitors
Haginaka, Kitabatake, Hirose et al
Anal. Biochem. (2013) 434 (1), 202-6 DOI: 10.1016/j.ab.2012.11.010
Haginaka, Kitabatake, Hirose et al
Anal. Biochem. (2013) 434 (1), 202-6 DOI: 10.1016/j.ab.2012.11.010
Cited by 14
AI Summary: OriGene’s recombinant Homo sapiens endothelial nitric oxide synthase (eNOS) was used in this study to confirm that cepharanthine competes for binding at the middle domain of Hsp90α, supporting the identification and characterization of Hsp90α inhibitors.
Snippet: Purified recombinant Homo sapiens endothelial nitric oxide synthase (eNOS) was 82 purchased from OriGene Technologies (Rockville, MD, USA). ... as shown in Table 1. Those values estimated by competition 204 and frontal chromatography studies are in good agreement.
Immunofluorescence microscopy to assess enzymes controlling nitric oxide availability and microvascular blood flow in muscle
Cocks, Shepherd, Shaw et al
Microcirculation (2012) 19 (7), 642-51 DOI: 10.1111/j.1549-8719.2012.00199.x
Cocks, Shepherd, Shaw et al
Microcirculation (2012) 19 (7), 642-51 DOI: 10.1111/j.1549-8719.2012.00199.x
Cited by 36
AI Summary: OriGene's purified human eNOS recombinant protein was used as a blocking control in immunofluorescence staining to validate antibody specificity in the assessment of endothelial nitric oxide synthase (eNOS) content and phosphorylation in muscle tissue.
Snippet: USA) was incubated overnight at 4°C with gentle agitation with a 20-fold excess of purified human eNOS recombinant protein (OriGene, Rockville, MD ... The quantification method showed a good reproducibility as demonstrated by the CV, which is lower than 10% for all analyses.
Showing 1-4 of 4 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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