VASH2 (NM_024749) Human Recombinant Protein

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SKU
TP308692M
Recombinant protein of human vasohibin 2 (VASH2), transcript variant 1, 100 µg
  $2,950.00
4 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC208692 protein sequence
Red=Cloning site Green=Tags(s)

MTGSAADTHRCPHPKGAKGTRSRSSHARPVSLATSGGSEEEDKDGGVLFHVNKSGFPIDSHTWERMWMHV
AKVHPKGGEMVGAIRNAAFLAKPSIPQVPNYRLSMTIPDWLQAIQNYMKTLHYLTNGQPSIERFPISFKT
YFSGNYFHHVVLGIYCNGRYGSLGMSRRAELMDKPLTFRTLSDLIFDFEDSYKKYLHTVKKVKIGLYVPH
EPHSFQPIEWKQLVLNVSKMLRADIRKELEKYARDMRMKILKPASAHSPTQVRSRGKSLSPRRRQASPPR
RLGRREKSPALPEKKVADLSTLNEVGYQIRI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 35.2 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity ELISA standard (PMID: 27702660)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_079025
Locus ID 79805
UniProt ID Q86V25
Cytogenetics 1q32.3
RefSeq Size 4362
RefSeq ORF 933
Summary Tyrosine carboxypeptidase that removes the C-terminal tyrosine residue of alpha-tubulin, thereby regulating microtubule dynamics and function (PubMed:29146869). Acts as an activator of angiogenesis: expressed in infiltrating mononuclear cells in the sprouting front to promote angiogenesis (PubMed:19204325).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins, Secreated Proteins
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Documents
Resources

file-alt Citations

Vasohibin 2 promotes human luminal breast cancer angiogenesis in a non-paracrine manner via transcriptional activation of fibroblast growth factor 2
Tu, Lu, Lv et al
Cancer Lett. (2016) 383 (2), 272-281 DOI: 10.1016/j.canlet.2016.09.031
AI Summary: OriGene’s VASH2 recombinant protein was used in this study to investigate the role of VASH2 in promoting angiogenesis in human luminal breast cancer.
Snippet: Concentrated CM (cCM;1 to 100) were prepared by ultrafiltration centrifugation(#UFC803096 and #UFC805096;Amicon Ultra-4, Millipore, USA) before WB and ELISA. TheVASH2 recombinant protein was purchased from Origene (Being, China). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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