Prothrombin (F2) (NM_000506) Human Recombinant Protein
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 67.5 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | MS digestion standard (PMID: 29702392) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_000497 |
| Locus ID | 2147 |
| UniProt ID | P00734 |
| Cytogenetics | 11p11.2 |
| RefSeq Size | 2018 |
| RefSeq ORF | 1866 |
| Synonyms | PT; RPRGL2; THPH1 |
| Summary | This gene encodes the prothrombin protein (also known as coagulation factor II). This protein is proteolytically cleaved in multiple steps to form the activated serine protease thrombin. The activated thrombin enzyme plays an important role in thrombosis and hemostasis by converting fibrinogen to fibrin during blood clot formation, by stimulating platelet aggregation, and by activating additional coagulation factors. Thrombin also plays a role in cell proliferation, tissue repair, and angiogenesis as well as maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in this gene lead to various forms of thrombosis and dysprothrombinemia. Rapid increases in cytokine levels following coronavirus infections can dysregulate the coagulation cascade and produce thrombosis, compromised blood supply, and organ failure. provided by RefSeq, May 2020 |
| Protein Categories | Cardiovascular diseases, Cytokines, Enzyme: Peptidases, Growth Factors, Intracellular Proteins, Secreated Proteins |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| Protein Pathways | Complement and coagulation cascades, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "Prothrombin" proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH308589 | F2 MS Standard C13 and N15-labeled recombinant protein (NP_000497) | 10 ug |
$3,360.00
|
|
| LC400182 | Lyophilized F2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY400182 | Transient overexpression lysate of coagulation factor II (thrombin) (F2) (as WB positive control) | 100 ug |
$436.00
|
|
| TP509466 | Purified recombinant protein of Mouse coagulation factor II (F2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug | 20 ug |
$1,020.00
|
|
| TP790103 | Purified recombinant protein of Human coagulation factor II (thrombin) (F2), esidues 44aa-end, with N-terminal His tag, expressed in human cells; | 50 ug |
$540.00
|
|
Citations
Fully validated SRM-MS-based method for absolute quantification of PIVKA-II in human serum: Clinical applications for patients with HCC
Sohn, Kim, Yeo et al
J Pharm Biomed Anal (2018) 156, 142-146 DOI: 10.1016/j.jpba.2018.04.025
Sohn, Kim, Yeo et al
J Pharm Biomed Anal (2018) 156, 142-146 DOI: 10.1016/j.jpba.2018.04.025
AI Summary: OriGene Technologies contributed recombinant prothrombin protein analogs (both unlabeled and ^13C^15N Arg/Lys-labeled forms) that were used as internal standards in the SRM-MS assay for accurate quantification of PIVKA-II in human serum.
Snippet: 0506-150-005). 2.2. Stable-isotope synthetic internal standard protein. Protein analogs that contained unlabeled and labeled ( 13 C 15 N Arg/Lys-labeled) recombinant prothrombin were purchased from Sino Biological Inc. (Beijing, China) and Origene Technologies
Showing 1-1 of 1 papers.
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