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Ribonuclease Inhibitor (RNH1) (NM_002939) Human Recombinant Protein

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SKU
TP308360
Recombinant protein of human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 1, 20 µg
Size:
20 ug 1 mg 100 ug
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC208360 protein sequence
Red=Cloning site Green=Tags(s)

MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGD
VGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDP
QCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCG
VTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVL
RAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISN
NRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVE
SVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 49.8 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity Cell treatment (PMID: 25889213)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_002930
Locus ID 6050
UniProt ID P13489
Cytogenetics 11p15.5
RefSeq Size 2057
RefSeq ORF 1383
Synonyms RAI; RNH
Summary Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 PubMed 3219362). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.supplied by OMIM, Jul 2010
Protein Categories Intracellular Proteins
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Other "Ribonuclease Inhibitor" proteins (29)
SKU Description Size Price
PH302451 RNH1 MS Standard C13 and N15-labeled recombinant protein (NP_976318) 10 ug
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PH303946 RNH1 MS Standard C13 and N15-labeled recombinant protein (NP_976322) 10 ug
$3,360.00
PH308360 RNH1 MS Standard C13 and N15-labeled recombinant protein (NP_002930) 10 ug
$3,360.00
PH313589 RNH1 MS Standard C13 and N15-labeled recombinant protein (NP_976319) 10 ug
$3,360.00
PH313642 RNH1 MS Standard C13 and N15-labeled recombinant protein (NP_976321) 10 ug
$3,360.00
PH318365 RNH1 MS Standard C13 and N15-labeled recombinant protein (NP_976320) 10 ug
$3,360.00
PH319235 RNH1 MS Standard C13 and N15-labeled recombinant protein (NP_976323) 10 ug
$3,360.00
LC401028 Lyophilized RNH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404324 Lyophilized RNH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404325 Lyophilized RNH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404326 Lyophilized RNH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404327 Lyophilized RNH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404328 Lyophilized RNH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404329 Lyophilized RNH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404330 Lyophilized RNH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401028 Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY404324 Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY404325 Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY404326 Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 4 (as WB positive control) 100 ug
$665.00
LY404327 Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 5 (as WB positive control) 100 ug
$665.00
LY404328 Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 6 (as WB positive control) 100 ug
$665.00
LY404329 Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 7 (as WB positive control) 100 ug
$436.00
LY404330 Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 8 (as WB positive control) 100 ug
$665.00
TP302451 Recombinant protein of human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 3, 20 µg 20 ug
$867.00
TP303946 Recombinant protein of human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 7, 20 µg 20 ug
$867.00
TP313589 Purified recombinant protein of Homo sapiens ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 4, 20 µg 20 ug
$867.00
TP313642 Recombinant protein of human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 6, 20 µg 20 ug
$867.00
TP318365 Purified recombinant protein of Homo sapiens ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 5, 20 µg 20 ug
$867.00
TP319235 Recombinant protein of human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 8, 20 µg 20 ug
$867.00

file-alt Citations

Migration towards SDF-1 selects angiogenin-expressing bone marrow monocytes endowed with cardiac reparative activity in patients with previous myocardial infarction
Ascione, Rowlinson, Avolio et al
Stem Cell Res Ther (2015) 6 (1), 53 DOI: 10.1186/s13287-015-0028-y
Cited by 15
AI Summary: In this study, OriGene’s recombinant human ribonuclease/angiogenin inhibitor 1 (RNH1) was used to investigate the role of angiogenin in cardiomyocyte survival by inhibiting its activity in conditioned medium during in vitro experiments.
Snippet: Synthetic angiogenin (BD, 10ng/mL) was used as a positive control. In inhibition experiments, recombinant human ribonuclease/angiogenin inhibitor 1 (RNH1, OriGene) was added to the conditioned medium, 1hr prior to incubation with cardiomyocytes. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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