RASA1 (NM_002890) Human Recombinant Protein
SKU
TP305473
Recombinant protein of human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1, 20 µg
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 116.2 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_002881 |
| Locus ID | 5921 |
| UniProt ID | P20936 |
| Cytogenetics | 5q14.3 |
| RefSeq Size | 4402 |
| RefSeq ORF | 3141 |
| Synonyms | CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP |
| Summary | The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. provided by RefSeq, May 2012 |
| Protein Categories | Cancer: Skin, Cytokines, Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance, MAPK signaling pathway |
Reviews
Documents
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "RASA1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH305473 | RASA1 MS Standard C13 and N15-labeled recombinant protein (NP_002881) | 10 ug |
$3,360.00
|
|
| LC401014 | Lyophilized RASA1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401014 | Transient overexpression lysate of RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Small Molecule KRAS Agonist for Mutant KRAS Cancer Therapy
Xu, Park, Magis et al
Mol. Cancer (2019) 18 (1), 85 DOI: 10.1186/s12943-019-1012-4
Xu, Park, Magis et al
Mol. Cancer (2019) 18 (1), 85 DOI: 10.1186/s12943-019-1012-4
AI Summary: OriGene’s purified recombinant RASA1 and RASGRP1 proteins were used in this study to investigate the activation and regulation of KRAS by the small molecule agonist KRA-533.
Snippet: Kit and Raf-1-RBD beads for pull-down of active RAS and Detection Kit were purchased from Thermo Fisher Scientific (Rockford, IL). Purified recombinant RASA1 and RASGRP1 proteins were purchased from OriGene Technologies (Rockville, MD). [γ-35S] GTP was purchased from PerkinElmer (Waltham, MA). Oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA). Chloroquines, 3-meth
Showing 1-1 of 1 papers.
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