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RBCK1 (NM_031229) Human Recombinant Protein

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SKU
TP303906L
Recombinant protein of human RanBP-type and C3HC4-type zinc finger containing 1 (RBCK1), transcript variant 2, 1 mg
Size:
1 mg 20 ug 100 ug
  $9,200.00
6 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
Recombinant protein was produced with TrueORF clone, RC203906. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag C-Myc/DDK
Predicted MW 57.4 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity Enzyme substrate (PMID: 26525107)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_112506
Locus ID 10616
UniProt ID Q9BYM8
Cytogenetics 20p13
RefSeq Size 2778
RefSeq ORF 1500
Synonyms C20orf18; HOIL-1; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; UBCE7IP3; XAP3; XAP4; ZRANB4
Summary The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. provided by RefSeq, Jul 2008
Protein Categories Immune system diseases, Intracellular Proteins
Protein Families Druggable Genome
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Documents
Resources

file-alt Citations

The paracaspase MALT1 cleaves HOIL1 reducing linear ubiquitination by LUBAC to dampen lymphocyte NF-κB signalling
Klein, Fung, Renner et al
Nat Commun (2015) 6, 8777 DOI: 10.1038/ncomms9777
Cited by 149
AI Summary: OriGene’s C-terminal Myc-FLAG-tagged full-length human HOIL1 protein was used in cleavage assays to demonstrate that MALT1 directly cleaves HOIL1, supporting the study’s findings on the regulation of linear ubiquitination and NF-κB signaling.
Snippet: …Cleavage of HOIL1 was analysed by 4–12% Bis-Tris SDS–polyacrylamide gel electrophoresis (PAGE) gradient gels (Life Technologies) and confirmed by immunoblotting using antibodies to N-HOIL1 (anti-N-terminal HOIL1;HPA024185;Sigma) and C-HOIL1 (anti-FLAG, clone M2, Sigma) cleavage products, respectively
WB
Showing 1-1 of 1 papers.
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