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CHI3L1 (NM_001276) Human Recombinant Protein

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SKU
TP303769
Recombinant protein of human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1), 20 µg
Size:
20 ug 1 mg 100 ug
  $867.00
4 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC203769 protein sequence
Red=Cloning site Green=Tags(s)

MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWE
WNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWL
YPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHG
AWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISG
PGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAM
VWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 42.4 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity Binding assay (PMID: 25688078)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_001267
Locus ID 1116
UniProt ID P36222
Cytogenetics 1q32.1
RefSeq Size 1867
RefSeq ORF 1149
Synonyms ASRT7; CGP-39; GP-39; GP39; HC-gp39; HCGP-3P; hCGP-39; YK-40; YKL-40; YKL40; YYL-40
Summary Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. provided by RefSeq, Sep 2009
Protein Categories Allergies and autoimmune diseases, Intracellular Proteins, Secreated Proteins
Protein Families Secreted Protein
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Documents
Resources
Other "CHI3L1" proteins (4)
SKU Description Size Price
PH303769 CHI3L1 MS Standard C13 and N15-labeled recombinant protein (NP_001267) 10 ug
$3,360.00
LC420034 Lyophilized CHI3L1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY420034 Transient overexpression lysate of chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1) (as WB positive control) 100 ug
$436.00
TP720379 Recombinant protein of human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1) 10 ug
$235.00

file-alt Citations

Chitinase 3-like 1: prognostic biomarker in clinically isolated syndromes
Cantó, Tintoré, Villar et al
Brain (2015)
Cited by 193
AI Summary: OriGene’s human recombinant CHI3L1 protein (TP303769) was used in blocking experiments to confirm the specificity of anti-CHI3L1 antibody staining in flow cytometry analyses in this study.
Snippet: …Representative examples of gating strategy and of anti-CHI3L1 and control isotype staining in the three cell subsets are shown in Supplementary Fig. 1. The specificity of anti-CHI3L1 antibody staining was assessed by means of blocking experiments incubating 1 mg of anti-CHI3L1 antibody with 2 mg of human recombinant CHI3L1 protein (TP303769 Origene) for 30 min at 4C before cell labelling, and resulted in a clear inhibition of CHI3L1 staining (Supplementary Fig. 2)
Showing 1-1 of 1 papers.
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