Alanine Transaminase (GPT) (NM_005309) Human Recombinant Protein
SKU
TP303756
Recombinant protein of human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), 20 µg
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 54.5 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_005300 |
| Locus ID | 2875 |
| UniProt ID | P24298 |
| Cytogenetics | 8q24.3 |
| RefSeq Size | 1896 |
| RefSeq ORF | 1488 |
| Synonyms | AAT1; ALT1; GPT1 |
| Summary | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.provided by RefSeq, Nov 2009 |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Metabolic pathways |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "Alanine Transaminase" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303756 | GPT MS Standard C13 and N15-labeled recombinant protein (NP_005300) | 10 ug |
$3,360.00
|
|
| LC417391 | Lyophilized GPT HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY417391 | Transient overexpression lysate of glutamic-pyruvate transaminase (alanine aminotransferase) (GPT) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity
Hu, Lausted, Yoo et al
Theranostics (2014) 4 (2), 215-228 DOI: 10.7150/thno.7868
Hu, Lausted, Yoo et al
Theranostics (2014) 4 (2), 215-228 DOI: 10.7150/thno.7868
Cited by 64
AI Summary: OriGene products, specifically purified human ALT1 and ALT2 recombinant proteins, were used as standards to calibrate enzymatic assays in this study, enabling accurate measurement of liver-specific blood proteins as biomarkers of hepatotoxicity.
Snippet: Recombinant protein standards were obtained from OriGene (Rockville, MD). ... For comparison, we calibrated our enzymatic assays using purified human ALT1 (0.46 IU/µg) and ALT2 (0.08 IU/µg) proteins (OriGene, Rockville, MD). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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