SHMT1 (NM_004169) Human Recombinant Protein

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SKU
TP303461
Recombinant protein of human serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 1, 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC203461 protein sequence
Red=Cloning site Green=Tags(s)

MTMPVNGAHKDADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLN
NKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMG
LDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLE
YARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKT
GKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIV
TGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQK
VAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASFFPLPGLPDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 52.9 kDa
Concentration >200 ug/mL as determined by Microplate Bradford Protein Assay method
Purity > 95% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_004160
Locus ID 6470
UniProt ID P34896
Cytogenetics 17p11.2
RefSeq Size 2553
RefSeq ORF 1449
Synonyms CSHMT; SHMT
Summary This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2013
Protein Categories Intracellular Proteins
Protein Pathways Cyanoamino acid metabolism, Glycine, Metabolic pathways, Methane metabolism, One carbon pool by folate, serine and threonine metabolism
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Other "SHMT1" proteins (7)
SKU Description Size Price
PH303461 SHMT1 MS Standard C13 and N15-labeled recombinant protein (NP_004160) 10 ug
$3,360.00
PH305102 SHMT1 MS Standard C13 and N15-labeled recombinant protein (NP_683718) 10 ug
$3,360.00
LC401341 Lyophilized SHMT1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407729 Lyophilized SHMT1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401341 Transient overexpression lysate of serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY407729 Transient overexpression lysate of serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP305102 Recombinant protein of human serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 2, 20 µg 20 ug
$867.00

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