SHMT1 (NM_004169) Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 52.9 kDa |
| Concentration | >200 ug/mL as determined by Microplate Bradford Protein Assay method |
| Purity | > 95% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004160 |
| Locus ID | 6470 |
| UniProt ID | P34896 |
| Cytogenetics | 17p11.2 |
| RefSeq Size | 2553 |
| RefSeq ORF | 1449 |
| Synonyms | CSHMT; SHMT |
| Summary | This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2013 |
| Protein Categories | Intracellular Proteins |
| Protein Pathways | Cyanoamino acid metabolism, Glycine, Metabolic pathways, Methane metabolism, One carbon pool by folate, serine and threonine metabolism |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "SHMT1" proteins (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303461 | SHMT1 MS Standard C13 and N15-labeled recombinant protein (NP_004160) | 10 ug |
$3,360.00
|
|
| PH305102 | SHMT1 MS Standard C13 and N15-labeled recombinant protein (NP_683718) | 10 ug |
$3,360.00
|
|
| LC401341 | Lyophilized SHMT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC407729 | Lyophilized SHMT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401341 | Transient overexpression lysate of serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY407729 | Transient overexpression lysate of serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| TP305102 | Recombinant protein of human serine hydroxymethyltransferase 1 (soluble) (SHMT1), transcript variant 2, 20 µg | 20 ug |
$867.00
|
|
Citations
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