RAF1 (NM_002880) Human Recombinant Protein
SKU
TP301983M
Recombinant protein of human v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1), 100 µg
$2,950.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 72.9 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_002871 |
| Locus ID | 5894 |
| UniProt ID | P04049 |
| Cytogenetics | 3p25.2 |
| RefSeq Size | 3291 |
| RefSeq ORF | 1944 |
| Synonyms | c-Raf; CMD1NN; CRAF; NS5; Raf-1 |
| Summary | This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. provided by RefSeq, Jul 2008 |
| Protein Categories | Cardiovascular diseases, Enzyme: Kinases, Growth Factors, Intracellular Proteins |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
ARAF protein kinase activates RAS by antagonizing its binding to RASGAP NF1
Su, Mukherjee, Yaeger et al
Mol Cell (2022) 82 (13), 2443-2457.e7 DOI: 10.1016/j.molcel.2022.04.034
Su, Mukherjee, Yaeger et al
Mol Cell (2022) 82 (13), 2443-2457.e7 DOI: 10.1016/j.molcel.2022.04.034
Cited by 23
AI Summary: OriGene products, including Recombinant Human ARAF (TP300737), BRAF (TP311013), CRAF (TP301983), and KRAS (TP301958), were used in this study to investigate how ARAF regulates RAS activation by displacing NF1 and influencing RAS signaling pathways.
Snippet: … We showed here that the Save;Recombinant Human ARAF Origene TP300737 Recombinant Human BRAF Origene TP311013 Recombinant Human CRAF Origene TP301983 Recombinant Human NF1-333 Creative Biomart NF1-334H Recombinant Human EGF Invitrogen PHG0311 Recombinant Rat NGF R & D 7185-NG-025 Recombinant Human KRAS Origene TP301958
The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2
Kovalski, Bhaduri, Zehnder et al
Mol. Cell (2019) 73 (4), 830-844.e12 DOI: 10.1016/j.molcel.2018.12.001
Kovalski, Bhaduri, Zehnder et al
Mol. Cell (2019) 73 (4), 830-844.e12 DOI: 10.1016/j.molcel.2018.12.001
AI Summary: In this study, OriGene’s recombinant proteins—including NRASQ61K (Cat# TP701004), NRAS (Cat# TP302681), MAPKAP1 (Cat# TP311745), mTOR (Cat# TP320457), KRASG12D (Cat# TP700052), HRASWT (Cat# TP316409), and custom-produced HRASG12V—were used to investigate and validate the interactions between oncogenic Ras proteins and mTORC2 components, elucidating key mechanisms in Ras-driven cancer proliferation.
Snippet: … They demonstrate that active Ras binds and stimulates mTORC2, representing a key dependency for Ras-driven proliferation genes and tumorigenesis. Cancer Ras Proteomics CRISPR mTOR MAPKAP1;... … They demonstrate that active Ras binds and stimulates mTORC2, representing a key dependency for Ras-driven proliferation genes and tumorigenesis. Cancer Ras Proteomics CRISPR mTOR MAPKAP1;NRASQ61K Human Recombinant Protein Origene Cat# TP701004 NRAS Human Recombinant Protein Origene Cat# TP302681 MAPKAP1 Human Recombinant Protein Origene Cat# TP311745 mTOR Human Recombinant Protein Origene Cat# TP320457 KRASG12D Human Recombinant Protein Origene Cat# TP700052 HRASWT Human Recombinant Protein Origene Cat# TP316409 HRASG12V Human Recombinant Protein Origene Custom Production from clone Cat# RC400129
Showing 1-2 of 2 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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