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SRP54 (NM_003136) Human Recombinant Protein

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SKU
TP301658M
Recombinant protein of human signal recognition particle 54kDa (SRP54), 100 µg
Size:
100 ug 20 ug 1 mg
  $2,508.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC201658 representing NM_003136
Red=Cloning site Green=Tags(s)

MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLN
KRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTF
RAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVAN
AIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHID
DFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILG
MIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQEL
LTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQG
AAGNMKGMMGFNNM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 55.5 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity ELISA capture for autoantibodies (PMID: 25524922)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_003127
Locus ID 6729
UniProt ID P61011
Cytogenetics 14q13.2
RefSeq Size 2164
RefSeq ORF 1512
Synonyms SCN8
Summary Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).UniProtKB/Swiss-Prot Function
Protein Categories Immune system diseases, Intracellular Proteins
Protein Pathways Protein export
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Documents
Resources

file-alt Citations

Anti-signal recognition particle autoantibody ELISA validation and clinical associations
Aggarwal, Oddis, Goudeau et al
Rheumatology (Oxford) (2014)
Cited by 43
AI Summary: OriGene’s recombinant, purified, full-length human SRP54 protein was used as the coating antigen in the ELISA developed and validated in this study to detect anti-SRP autoantibodies in myositis patients.
Snippet: Development of the anti-SRP antibody ELISA. Recombinant, purified, full-length human SRP54 (Origene Technologies, Rockville, MD, USA) was coated (100 ng/well) on a 96-well high-binding ELISA plate (Costar, Corning, NY, USA). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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