DDX5 (NM_004396) Human Recombinant Protein
$2,950.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 69 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | Phosphorylation substrate (PMID: 25649741) Binding assay (PMID: 27148684) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004387 |
| Locus ID | 1655 |
| UniProt ID | P17844 |
| Cytogenetics | 17q23.3 |
| RefSeq Size | 3769 |
| RefSeq ORF | 1842 |
| Synonyms | G17P1; HLR1; HUMP68; p68 |
| Summary | This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2017 |
| Protein Categories | Intracellular Proteins |
| Protein Pathways | Spliceosome |
Reviews
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Resources
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Citations
Glutamine Deprivation Triggers Tribbles Homolog 3 Dependent G-Quadruplex Resolution to Maintain DNA Repair and Tumor Survival
Ji, Sun, Sun et al
Adv Sci (Weinh) (2026)
Ji, Sun, Sun et al
Adv Sci (Weinh) (2026)
AI Summary: OriGene products, specifically recombinant TRIB3-His (Cat# AR51742PU) and DDX5-FLAG (Cat# TP300371) proteins, were used in biochemical assays to study the molecular interaction and functional roles of TRIB3 and DDX5 in G-quadruplex DNA resolution and DNA repair mechanisms in hepatocellular carcinoma cells.
DDX5-targeting fully human monoclonal autoantibody inhibits proliferation and promotes differentiation of acute promyelocytic leukemia cells by increasing ROS production
Wu, You, Ma et al
Cell Death Dis (2020) 11 (7), 552 DOI: 10.1038/s41419-020-02759-5
Wu, You, Ma et al
Cell Death Dis (2020) 11 (7), 552 DOI: 10.1038/s41419-020-02759-5
Cited by 19
AI Summary: In this study, the DDX5 protein from OriGene was used to evaluate the specific binding and affinity of the newly developed anti-DDX5 human monoclonal autoantibody (2F5), supporting the characterization of its therapeutic potential against acute promyelocytic leukemia cells.
Snippet: One stable clone secreting anti-DDX5 human monoclonal autoantibody was obtained and named after 2F5. The specific binding and affinity between 2F5 and DDX5 (OriGene, Rockville, USA) was determined by Surface Plasmon Resonance (Biacore X100, GE, USA;- One stable clone secreting anti-DDX5 human monoclonal autoantibody was obtained and named after 2F5. The specific binding and affinity between 2F5 and DDX5 (OriGene, Rockville, USA) was determined by Surface Plasmon Resonance (Biacore X100, GE, USA
ATAD5 restricts R-loop formation through PCNA unloading and RNA helicase maintenance at the replication fork
Kim, Kang, Park et al
Nucleic Acids Res. (2020) 48 (13), 7218-7238 DOI: 10.1093/nar/gkaa501
Kim, Kang, Park et al
Nucleic Acids Res. (2020) 48 (13), 7218-7238 DOI: 10.1093/nar/gkaa501
Cited by 68
Resveratrol directly targets DDX5 resulting in suppression of the mTORC1 pathway in prostate cancer
Taniguchi, Iizumi, Watanabe et al
Cell Death Dis (2016) 7, e2211 DOI: 10.1038/cddis.2016.114
Taniguchi, Iizumi, Watanabe et al
Cell Death Dis (2016) 7, e2211 DOI: 10.1038/cddis.2016.114
Cited by 54
A Novel Anti-Cancer Agent, 1-(3, 5-Dimethoxyphenyl)-4-[(6-fluoro-2-methoxyquinoxalin-3-yl)aminocarbonyl] piperazine (RX-5902),Interferes with β-Catenin Function through Y593 phospho-p68RNA Helicase
Kost, Yang, Li et al
J. Cell. Biochem. (2015)
Kost, Yang, Li et al
J. Cell. Biochem. (2015)
Cited by 41
AI Summary: In this study, recombinant p68 protein from OriGene was used to investigate the molecular mechanism by which the anti-cancer agent RX-5902 interferes with β-catenin function via Y593 phospho-p68 RNA helicase.
Snippet: antibody were obtained from Cell Signaling (Danvers, MA). Recombinant Proteins Recombinant β-catenin and p68 protein were purchased from Creative Biomart (Shirley, NY) and Origene (Rockville, MD), respectively.Recombinant β-catenin was used without further ... Cite SaveSaving
Showing 1-5 of 5 papers.
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