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DBR1 (NM_016216) Human Recombinant Protein

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SKU
TP300024L
Recombinant protein of human debranching enzyme homolog 1 (S. cerevisiae) (DBR1), 1 mg
Size:
1 mg 20 ug 100 ug
  $7,820.00
6 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC200024 protein sequence
Red=Cloning site Green=Tags(s)

MRVAVAGCCHGELDKIYETLALAERRGPGPVDLLLCCGDFQAVRNEADLRCMAVPPKYRHMQTFYRYYSG
EKKAPVLTLFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRIGGISGIFKSHDYRKGHFECP
PYNSSTIRSIYHVRNIEVYKLKQLKQPIDIFLSHDWPRSIYHYGNKKQLLKTKSFFRQEVENNTLGSPAA
SELLEHLKPTYWFSAHLHVKFAALMQHQAKDKGQTARATKFLALDKCLPHRDFLQILEIEHDPSAPDYLE
YDIEWLTILRATDDLINVTGRLWNMPENNGLHARWDYSATEEGMKEVLEKLNHDLKVPCNFSVTAACYDP
SKPQTQMQLIHRINPQTTEFCAQLGIIDINVRLQKSKEEHHVCGEYEEQDDVESNDSGEDQSEYNTDTSA
LSSINPDEIMLDEEEDEDSIVSAHSGMNTPSVEPSDQASEFSASFSDVRILPGSMIVSSDDTVDSTIDRE
GKPGGTVESGNGEDLTKVPLKRLSDEHEPEQRKKIKRRNQAIYAAVDDDDDDAA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 61.4 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_057300
Locus ID 51163
UniProt ID Q9UK59
Cytogenetics 3q22.3
RefSeq Size 2690
RefSeq ORF 1632
Summary The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. provided by RefSeq, Nov 2011
Protein Categories Intracellular Proteins, Nervous system Diseases
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Documents
Resources

file-alt Citations

Recording gene expression order in DNA by CRISPR addition of retron barcodes
Bhattarai-Kline, Lear, Fishman et al
Nature (2022) 608 (7921), 217-225 DOI: 10.1038/s41586-022-04994-6
Cited by 90
AI Summary: In this study, the OriGene DBR1 (TP300024) enzyme was used to treat purified DNA samples, contributing to the processing of nucleic acids during the experimental workflow for recording gene expression order in DNA.
Snippet: … Here we report a Save;a plasmid midiprep kit (Qiagen, 12943). The purified DNA was then treated for 30 min at 37 °C with RNase A/T1 mix (Thermo Fisher, EN0551) and, if required, DBR1 (OriGene, TP300024) and/or RecJf (NEB, M0264). …
Showing 1-1 of 1 papers.
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