Tumor necrosis factor (TNF-alpha) (77-233) Human Protein
Tumor necrosis factor (TNF-alpha) (77-233) human recombinant protein, 0.5 mg
|Description||Tumor necrosis factor (TNF-alpha) (77-233) human recombinant protein, 0.5 mg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL|
|Predicted MW||17.5 kDa|
|Purity||>95% by SDS-PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: PBS, pH 7.4
|Bioactivity||Biological: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect
is < 0.16 ng/ml.
|Endotoxin||< 1.0 EU per 1 μg of protein (determined by LAL method)|
|Preparation||Liquid purified protein|
|Applications||Protocol: Activity Assay
1. L929 cytotoxicity assay using actinomycin D
2. Cell Number: 2 x 10e4 cells/well
3. Incubation: 24hr after sample treatment
4. Assay Media: 5% FBS media
5. Cytokine Concentration: 12 pg/ml - 25 ng/ml
6. Detection method : MTT assay
|Protein Description||The active form of this protein is a trimer. Recombinant human TNF-α was expressed in E. coli. Additional amino acid, Methionine, was attached at N-terminus of the protein.|
|Storage||Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F|
|Summary||This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]|
|Protein Families||Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane|
|Protein Pathways||Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.