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groS / groES (1-97) E.coli Protein

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SKU
SA6021
Recombinant e.coli GroES protein
Size:
100 ug
  $355.00
3 Weeks*
Bulk/Customize

Other products for "NP_418566"

Specifications
Specifications
Product Data
Species E.coli
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA
Predicted MW 10.4 kDa (97aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 5mM DTT, 10%glycerol
Preparation Liquid protein
Recommended Dilution SDS-PAGE
Protein Description This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_418566
UniProt ID P0A6F9
Synonyms CPN10, GroES, 10 kDa chaperonin, groS, mopB, 10 kDa heat shock protein mitochondrial, Chaperonin 10, Cpn 10, Early pregnancy factor, EPF, Heat shock 10kD protein 1 chaperonin 10, Heat shock 10kDa protein 1, Heat shock 10kDa protein 1 chaperonin 10, HSP 10, HSP10, HSPE 1, HSPE1
Summary GroES protein is the co-chaperonin of GroES in E. coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques.
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Documents
Resources

file-alt Citations

N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration
Chen, Melo, Jakob et al
Matrix Biol. (2018)
AI Summary: OriGene products, specifically the recombinant proteins HtrA1S328A (TP700208) and HtrA1WT (TP322362), were used in this study to assess HtrA1's cleavage activity and specificity toward thrombospondin-1, contributing to the identification of a proangiogenic fragment relevant to age-related macular degeneration.
Snippet: ;Specificity profiling was performed as described elsewhere [59, 72]. In brief, proteomederived peptide libraries were generated using Hek293T cell lysate digested with either trypsin or GluC [72]. Recombinant proteins of HtrA1S328A and HtrA1WT were purchased from Origene (TP700208, TP322362)
Showing 1-1 of 1 papers.
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