groS / groES Escherichia coli Protein

CAT#: SA6021

groS / groES e. coli protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Escherichia coli
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA
• Predicted MW: 10.4 kDa
• Concentration: lot specific
• Purity: >95% pure by SDS-PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid protein
  Buffer System: 25 mM Tris-HCl (pH 7.5) 100 mM NaCl, 5 mM DTT, 10% Glycerol
• Preparation: Liquid protein
• Protein Description: This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: GroES protein is the co-chaperonin of GroES in E.coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E.coli and cloned into an expression vector. This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques.