Protein A (Sepharose) Staphylococcus aureus Protein

CAT#: RA100S2

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Protein A (Sepharose) s. aureus protein, 2 ml


Product Datasheet for 'RA100S2'


USD 345.00


Availability*
5 Days

Size
    • 2 ml


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Specifications

Product Data
Description Protein A (Sepharose) s. aureus protein, 2 ml
Species Staphylococcus aureus
Concentration 6.0 mg Protein A per cc of drained Sepahrose gel
Buffer State: Suspension of Sepharose beads (~2.0 cc suspension per cc settled beads).
Buffer System: 20% (v/v) Ethanol
Label: Sepharose (TM)
Presentation Label: Sepharose
Bioactivity Specific: ~35 mg Human IgG bound per cc of drained beads.
Preparation Suspension of Sepharose beads (~2.0 cc suspension per cc settled beads).
Applications Ideal for laboratory and process scale recovery and purification of monoclonal antibodies from dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments.  This product is suitable for use at 20 ul per immunoprecipitation reaction
Protein Description Native Protein A (Staphylococcus aureus) coupled to bead by cyanogen bromide activation method.
Note Matrix: Sepharose 4 Fast Flow, highly cross linked, 4% agarose derivative.
Bead Size: 45-165 µm in size when swollen.
Protein A/Sepharose: 6.0 mg Protein A per cc of drained sepharose gel.
Binding Capacity: Approximately 35 mg Human IgG per cc of drained sepharose gel.
Exclusion Limit (Mr): 3 x 10e7
Maximum Linear Flow Rate: < 1300 cm/hr
pH Stability: Long term stability within pH range of 3 to 9. Short term stability within pH range of 2 to 10.

Applications: Ideal for laboratory and process scale recovery and purification of monoclonal antibodies from dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes.
Storage Store vial at 2-8°C prior to opening.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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