Interleukin-1 beta / IL-1B Human Protein

SKU DA3558

Interleukin-1 beta / IL-1B human recombinant protein, 10 µg

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQANMP VFLGGTKGGQ DITDFTMQFV SS
• Predicted MW: 17.0 kDa
• Purity: >98% pure by SDS-PAGE and Silver stain
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Lyophilized purified protein; Buffer System: PBS; Stabilizer: None
• Bioactivity: Biological: Measured in a cell proliferation assay using murine D10G4.1 cells. The ED50 for this effect is typically 2-10 pg/ml.
• Endotoxin: < 0.1 ng per µg (IEU/µg) of rh IL-1beta
• Reconstitution Method: The lyophilized rh IL-1beta is soluble in water and most aqueous buffers.; The lyophilized powder can be restored in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
• Preparation: Lyophilized purified protein
• Protein Description: Recombinant Human Interleukin-1beta produced in E.Coli is a non-glycosylated, Interleukin-1 beta Polypeptide chain containing 153 amino acids and having a molecular mass of 17.0 kDa. ; Result by N-terminal sequencing: APVRSL and MAPVRS
• Note: Range: 0.1-10.0 ng/ml
• Storage: Store lyophilized at RT for 3 weeks or (preferably in a desiccator) at -20°C for longer.; Following reconstitution store the antibody undiluted at 2-8°C for one week; or (in aliquots) at -20°C for longer.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA); Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient

Reference Data

• RefSeq: NP_000567
• Locus ID: 3553
• UniProt ID: P01584
• Cytogenetics: 2q14.1
• Synonyms: IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin
• Summary: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2020]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Alzheimer's disease, Apoptosis, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway, Type I diabetes mellitus