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Interleukin-6 / IL6 Human Protein

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SKU
DA3547
Interleukin-6 / IL6 human recombinant protein, 20 µg
Size:
20 ug 5 ug
  $300.00
2 Weeks*
Bulk/Customize

Other products for "IL6 or NM_000600"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Predicted MW 21 kDa
Purity >98% pure by RP-HPLC, Anion-exchange FPLC, Silverstain.
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized purified protein.
Buffer System: PBS
Stabilizer: None
Bioactivity Biological: The ED50 as determined by dose-dependent stimulation of murine hybridoma B9 cells is in the range of 2-10 pg/ml.
Endotoxin < 0.1 ng per µg (IEU/µg) of rh IL-6
Reconstitution Method The lyophilized IL-6 is soluble in water and most aqueous buffers. It should be reconstituted in water to a concentration not less than 0.1 mg/ml.
This solution can then be stored at 2-8°C for future use or diluted into other buffered solutions. Further dilutions should be made into buffer containing carrier protein or medium containing serum.
Preparation Lyophilized purified protein.
Protein Description Recombinant human IL-6 produced in E. coli is a single, non-glycosylated polypeptide containing 184 amino acid residues.
Storage The lyophilized IL-6, although stable at room temperature for 3 weeks, is best stored desiccated at -20°C.
Reconstituted Il-6 should be stored in working aliquots at -20°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Reference Data
RefSeq NP_000591
Locus ID 3569
UniProt ID P05231
Cytogenetics 7p15.3
Synonyms IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF
Summary This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). provided by RefSeq, Aug 2020
Protein Categories Allergies and autoimmune diseases, Chemokines, Cytokines, Growth Factors, Intracellular Proteins, Membrane Proteins, Secreated Proteins
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Jak-STAT signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prion diseases, Toll-like receptor signaling pathway
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Documents
Resources

file-alt Citations

Corinthian Currants Supplementation Restores Serum Polar Phenolic Compounds, Reduces IL-1beta, and Exerts Beneficial Effects on Gut Microbiota in the Streptozotocin-Induced Type-1 Diabetic Rat
Kompoura, Prapa, Vasilakopoulou et al
Metabolites (2023) 13 (3) DOI: 10.3390/metabo13030415
Cited by 15
AI Summary: OriGene products, specifically the rat IL-1 beta ELISA kit (EA100264) and rat IL-6 ELISA kit (EA100168), were used in this study to measure inflammatory markers and assess the effects of Corinthian currant supplementation on inflammation in diabetic rats.
Snippet: y). Inflammatory markers, i.e., interleukin-1beta (IL-1b) and interleukin-6 (IL-6), were also determined by a sandwich ELISA method (rat IL-1 beta ELISA kit EA100264, and rat IL-6 ELISA kit EA100168, OriGene, Rockville, MD, USA) at the end of the intervention. 2.4.2. Determination of Polar Phenols in Rat Serum Polar Phenol Isolation from Serum Samples For the extraction of polar phenols, rat seru
FigureFigureFigure
Microglia Regulate Cell Genesis in a Sex-dependent Manner in the Neonatal Hippocampus
Nelson, Peketi, Lenz
Neuroscience (2021) 453, 237-255 DOI: 10.1016/j.neuroscience.2020.10.009
Cited by 23
AI Summary: In this study, OriGene products, specifically the Il1b (Cat# RQ025866) and Tnf (Cat# RQ025548) qPCR primers, were used to measure gene expression changes in the hippocampus following microglia depletion, helping to reveal the role of microglia in regulating cell genesis in a sex-dependent manner.
Latifolin protects against myocardial infarction by alleviating myocardial inflammatory via the HIF-1α/NF-κB/IL-6 pathway
Lai, Zhang, Chen et al
Pharm Biol (2020) 58 (1), 1156-1166 DOI: 10.1080/13880209.2020.1840597
Cited by 27
AI Summary: OriGene products, specifically the HIF1A (TA500073) and IL6 (TA326561) antibodies, were used for western blot analysis to measure protein expression levels and elucidate the molecular mechanisms by which latifolin alleviates myocardial inflammation in this study.
FigureFigureFigure
Kaposi's Sarcoma-associated Herpesvirus Viral Interleukin 6 Signaling Upregulates Integrin Beta 3 Levels and is Dependent on STAT3
Rivera-Soto, Dissinger, Damania
J. Virol. (2019)
AI Summary: OriGene contributed to this study by providing the human IL-6 antibody (TA300413), which was used to detect and analyze IL-6 protein levels in experiments investigating the regulation of integrin β3 by viral interleukin-6 signaling.
Snippet: … Human IL-6 antibodies were obtained from Origene 411 (TA300413) and CST (12153S). … Actin (sc-47778) antibody conjugated with HRP was purchased from 414 Santa Cruz Biotechnology
Showing 1-4 of 4 papers.
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