Interleukin-4 / IL4 Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Predicted MW | 15 kDa |
| Purity | >98% pure by SDS-PAGE and HPLC analyses. |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized purified protein. Buffer System: PBS without stabilizer. |
| Bioactivity | Biological: Recombinant Human IL-4 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is 0.1-0.3 ng/ml. For most in vitro applications, IL-4 exerts its biological activity in the concentration range of 0.1 to 10.0 ng/ml. Specific: 5 x 106 units/mg |
| Endotoxin | < 0.1 ng per µg (IEU/µg) of IL-4 |
| Reconstitution Method | The lyophilized IL-4 is soluble in water and most aqueous buffers. Restore in water to a concentration of 100 ng/ml. This solution can be diluted into water or other buffered solutions or stored at -20°C for future use. |
| Preparation | Lyophilized purified protein. |
| Protein Description | Recombinant Human IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain having a molecular mass of 14.9 kDa and containing 129 amino acid residues. |
| Note | Centrifuge vial before opening! |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_000580 |
| Locus ID | 3565 |
| UniProt ID | P05112 |
| Cytogenetics | 5q31.1 |
| Synonyms | IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra |
| Summary | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. provided by RefSeq, Aug 2020 |
| Protein Categories | Allergies and autoimmune diseases, Cytokines, Growth Factors, Secreated Proteins |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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