Heat shock factor 1 / HSF1 (1-529, His-Tag) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 59.4 kDa (549aa) |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 80% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE |
| Protein Description | Heat shock factor 1 (HSF1) is a major transactivator of heat shock proteins in response to environmental changes, and it is also involved in oogenesis, spermatogenesis, and placental development. Best known for its involvement in heat shock response, Hsf1p regulates the transcription of hundreds of targets, including genes involved in protein folding, detoxification, energy generation, carbohydrate metabolism, and cell wall organization. Recombinant HSF1, fused to His tag, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_005517.1 |
| Locus ID | 3297 |
| UniProt ID | Q00613 |
| Cytogenetics | 8q24.3 |
| Synonyms | Heat shock factor 1, Heat shock transcription factor 1(HSTF), Heat shock factor 1, HSF1, Heat shock factor 1 Heat shock factor protein 1, Heat shock transcription factor 1, HSTF 1. |
| Summary | The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. provided by RefSeq, Jul 2017 |
| Protein Categories | Intracellular Proteins, Transciption Factors |
| Protein Families | SDS-PAGE |
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