NGF Mouse Protein

Name: NGF Mouse Protein
Brand: OEM
SKU: SA003
Price: 1810 USD
Availability: InStock

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SKU

SA003

NGF mouse protein, 1 mg

1 Citation

Datasheet SDS

$Published customer image: Mouse Nerve Growth Factor 2.5S (SA003) used in vitro to stimulate the differentiation of PC12 cells. Image caption: SB203580 increases lysosomal localization and degradation of αSyn and modulates lysosomal distribution and function. A, NGF-differentiated PC12-αSyn/p25α cells were treated with 1 μM SB203580 and protease inhibitors E64 (10 μM) and LP (50/67 μg/ml, respectively) for 2 days before indirect immunofluorescence to reveal αSyn (LB509 mAb) and LAMP-1. Note increased localization of αSyn in LAMP1-positive compartments in SB203580-treated cells (arrows). The images are representative of two independent experiments. Bar represents 10 μm. B, NGF-differentiated PC12-αSyn/p25α cells were cultured 2 days with or without 1 μM SB203580 before homogenization and fractionation of equal protein loads by sucrose gradient centrifugation. Collected fractions were analyzed by Western blotting for αSyn, cathepsin D, and LC3. Note the increased distribution of &apha;Syn and cathepsin D in heavy lysosomal fractions in SB203580-treated cells. The bands in the LC3 blot represent LC3-I (upper band) and LC3-II (lower band). The shown blots are representative of two independent experiments.br> From: Borland H, Rasmussen I, Bjerregaard-Andersen K, Rasmussen M, Olsen A, Vilhardt F. α-synuclein buildup is alleviated via ESCRT-dependent endosomal degradation brought about by p38MAPK inhibition in cells expressing p25α. <a href="https://pubmed.ncbi.nlm.nih.gov/36162505/"target="_blank" rel="noopener">J Biol Chem. 2022 Nov;298(11):102531</a>. doi: 10.1016/j.jbc.2022.102531. This image is from an open access article distributed under terms of a <a href="https://creativecommons.org/licenses/by/4.0/"target="_blank" rel="noopener">Creative Commons Attribution License</a>.$

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Specifications

Specifications
Product Data
Species	Mouse
Protein Source	Submax. Glands
Predicted MW	13 kDa
Concentration	lot specific
Purity	>98% pure by SDS-PAGE.
Buffer	State: Lyophilized purified protein.
Reconstitution Method	Restore with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Preparation	Lyophilized purified protein.
Recommended Dilution	Functional Assays.
Protein Description	Purified Natural Murine Nerve Growth Factor. Preparation: Murine nerve growth factor is prepared from the submaxillary glands of mice by a modification of the method of Bocchini and Angeletti.
Storage	Store the antibody undiluted at 2-8°C prior to reconstitution and at -20°C following reconstitution. Avoid repeated freezing and thawing.
Stability	Shelf life: Six three from despatch.
Shipping	Blue Ice
Reference Data
RefSeq	NP_002497
Locus ID	4803
Cytogenetics	1p13.2
Synonyms	Beta-NGF, NGFB,Beta-nerve growth factor
Summary	This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. provided by RefSeq, Jul 2008
Protein Categories	Growth Factors, Intracellular Proteins, Nervous system Diseases, Secreated Proteins
Protein Families	Functional Assays.
Protein Pathways	Apoptosis, MAPK signaling pathway, Neurotrophin signaling pathway

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Resources

Recombinant Protein Resources
Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

Citations

Bladder Transplantation of Amniotic Fluid Stem Cell may Ameliorate Bladder Dysfunction After Focal Cerebral Ischemia in Rat
Liang, Shaw, Huang et al
Stem Cells Transl Med (2017) 6 (4), 1227-1236 DOI: 10.1002/sctm.16-0212

Cited by 24

AI Summary: In this study, OriGene's NGF (Cat# SR321060), CHRM2 (Cat# SR506434), CHRM3 (Cat# SR506435), and P2RX1 (Cat# SR506436) qPCR primer sets were used to measure gene expression changes in rat bladders, helping to assess the molecular effects of amniotic fluid stem cell transplantation after cerebral ischemia.

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