Goat IgG (Fc Fragment) Goat Protein
Specifications
| Product Data | |
| Species | Goat |
|---|---|
| Protein Source | Serum |
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(ab’)2 or anti-Papain. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Preparation | Lyophilized purified Ig fraction. |
| Protein Description | Biotin Conjugated Goat IgG F(c) fragment |
| Note | Goat IgG F(c) fragment Biotin conjugated has been tested by SDS-PAGE and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | Goat IgG F(c) fragment Biotin conjugation, Goat IgG Fc fragment BAC conjugate |
| Summary | Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This antibody possesses the F(c) region, recognized by high-affinity Fc receptor proteins. |
| Protein Families | anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(ab')2 or anti-Papain., ELISA, Immunoblotting, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin |
Reviews
Documents
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Resources
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Citations
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