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Mouse IgG (Fc specific) Mouse Protein

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SKU
RA106
Mouse IgG Fc Biotin
Size:
1 mg
  $790.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Mouse
Protein Source Serum
Concentration 1.0 mg/mL by UV absorbance at 280 nm
Purity This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum.  No reaction was observed against anti-Mouse IgG F(ab’)2  or anti-Papain.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Preparation Lyophilized purified protein
Protein Description Mouse IgG F(c) fragment Biotin conjugated.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum.
No reaction was observed against anti-Mouse IgG F(ab')2 or anti-Papain.
Note Mouse IgG F(c) fragment has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Reference Data
Synonyms Mouse IgG F(c) Fragment Biotin Conjugation, Mouse IgG Fc Fragment Biotin Conjugated
Summary Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
Protein Families Immunoblotting.
ELISA.
Immunohistochemistry.
Immunomicroscopy.
as well as other antibody based assays using streptavidin or avidin conjugates.
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Documents
Resources

file-alt Citations

CELL DEATH. Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53
Yoon, Byun, Kwon et al
Science (2015) 349 (6247), 1261669 DOI: 10.1126/science.1261669
Cited by 235
AI Summary: OriGene's pCMV6-AC-FC vector was used to generate Fc-tagged soluble DD1α proteins (DD1α-Ig), enabling the study of DD1α's role in apoptotic cell clearance in this research.
Snippet: For Fc-tagged soluble DD1a proteins (DD1a-Ig), the extracellular region of DD1a was cloned upstream of the mouse IgG-Fc region (DD1a-Ig) in the pCMV6-AC-FC vector (OriGene, Rockville, MD). P
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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