Avidin Chicken Protein
Specifications
| Product Data | |
| Species | Chicken |
|---|---|
| Protein Source | Egg white |
| Concentration | lot specific |
| Purity | This product was prepared from chromatographically pure avidin followed by extensive dialysis against the buffer stated below. |
| Conjugation | HRP |
| Buffer | State: Lyophilized purified fraction. Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: Horseradish Peroxidase (HRP) conjugated. Presentation Label: HRP |
| Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
| Preparation | Lyophilized purified fraction. |
| Recommended Dilution | Suitable for Immunoblotting (Western: 1/2,000-1/10,000, or Dot blot), ELISA (1/100,000; This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) as a substrate for 30 minutes at room temperature), immuno-peroxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-biotin-avidin based enzymatic assays. |
| Protein Description | Avidin Peroxidase Conjugate. |
| Note | Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Peroxidase. Caution: Do Not Add Sodium Azide |
| Storage | Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_990651 |
| Locus ID | 396260 |
| Synonyms | AVD |
| Summary | avidin |
| Protein Families | 000, 000) as well as other peroxidase-biotin-avidin based enzymatic assays., 000-1/10, 000-1/5, 000; This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using ABTS (2, 2'-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) as a substrate for 30 minutes at room temperature), ELISA (1/100, immuno-peroxidase electron microscopy and Immunohistochemistry (1/1, or Dot blot), Suitable for Immunoblotting (Western: 1/2 |
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