Human IgG (F(c) Fragment) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Protein Source | Serum |
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | Human IgG F(c) fragment Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Preparation | Lyophilized purified serum |
| Recommended Dilution | ELISA,Immunohistochemistry,Western Blot |
| Protein Description | This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti- Human IgG F(ab')2 or anti-Papain. |
| Note | Human IgG F(c) fragment Biotin conjugated has been tested in dot blot and can be utilized as a control reagent in both Western Blotting and ELISA format experiments. |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Human IgG F(c) fragment Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| Locus ID | 109864281 |
| Cytogenetics | 21p11.2 |
| Synonyms | Human Immunoglobulin G F(c) fragment, IgG Fc, Vitamin H, Coenzyme R |
| Summary | 45S ribosomal DNA (rDNA) arrays, or clusters, are present on human chromosomes 13, 14, 15, 21 and 22, designated RNR1 through RNR5, respectively. Each cluster consists of multiple 45S rDNA repeat units that vary in number among individuals and chromosomes, with total diploid copy number estimates ranging from 60 to >800 repeat units in a human genome. The 45S rDNA repeat unit encodes a 45S rRNA precursor, transcribed by RNA polymerase I, which is processed to form the 18S, 5.8S and 28S rRNAs. This gene represents a copy of the 5.8S ribosomal RNA on chromosome 21. provided by RefSeq, Mar 2017 |
| Protein Families | ELISA, IHC, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates., Suitable for use in immunoblotting |
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