Human IgG (F(c) Fragment) Human Protein

SKU
RA036B
Human IgG Fc Biotin
  $600.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Protein Source Serum
Concentration 1.0 mg/mL by UV absorbance at 280 nm
Purity Human IgG F(c) fragment Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum.  No reaction was observed against anti-Human IgG F(ab’)2  or anti-Papain.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Preparation Lyophilized purified serum
Recommended Dilution ELISA,Immunohistochemistry,Western Blot
Protein Description This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti- Human IgG F(ab')2 or anti-Papain.
Note Human IgG F(c) fragment Biotin conjugated has been tested in dot blot and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Human IgG F(c) fragment Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Reference Data
Locus ID 109864281
Cytogenetics 21p11.2
Synonyms Human Immunoglobulin G F(c) fragment, IgG Fc, Vitamin H, Coenzyme R
Summary 45S ribosomal DNA (rDNA) arrays, or clusters, are present on human chromosomes 13, 14, 15, 21 and 22, designated RNR1 through RNR5, respectively. Each cluster consists of multiple 45S rDNA repeat units that vary in number among individuals and chromosomes, with total diploid copy number estimates ranging from 60 to >800 repeat units in a human genome. The 45S rDNA repeat unit encodes a 45S rRNA precursor, transcribed by RNA polymerase I, which is processed to form the 18S, 5.8S and 28S rRNAs. This gene represents a copy of the 5.8S ribosomal RNA on chromosome 21. provided by RefSeq, Mar 2017
Protein Families ELISA, IHC, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates., Suitable for use in immunoblotting
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products