Bovine IgG Bovine Protein
Specifications
| Product Data | |
| Species | Bovine |
|---|---|
| Protein Source | Serum |
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine IgG, anti-Bovine Serum and anti-Alkaline Phosphatase (calf intestine). |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Preparation | Liquid |
| Protein Description | Bovine IgG |
| Note | Bovine IgG whole molecule alkaline phosphatase conjugation can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | Bovine IgG Alkaline Phosphatase conjugation, Bovine IgG alk phos conjugation, Alkaline phosphatase conjugated Bovine IgG, alk phos conjugated Bovine IgG |
| Summary | Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. |
Reviews
Documents
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Resources
| Recombinant Protein Resources |
Citations
Schön, Breithaupt, Höper et al
PLoS Pathog (2021) 17 (4), e1009490 DOI: 10.1371/journal.ppat.1009490
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