Bovine IgG Bovine Protein

SKU
RA022AP
Bovine IgG Alkaline Phosphatase
  $365.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Bovine
Protein Source Serum
Concentration 1.0 mg/mL by UV absorbance at 280 nm
Purity This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine IgG, anti-Bovine Serum and anti-Alkaline Phosphatase (calf intestine).
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Preparation Liquid
Protein Description Bovine IgG
Note Bovine IgG whole molecule alkaline phosphatase conjugation can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Reference Data
Synonyms Bovine IgG Alkaline Phosphatase conjugation, Bovine IgG alk phos conjugation, Alkaline phosphatase conjugated Bovine IgG, alk phos conjugated Bovine IgG
Summary Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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Documents
Resources

file-alt Citations

Neuraminidase-associated plasminogen recruitment enables systemic spread of natural avian Influenza viruses H3N1
Schön, Breithaupt, Höper et al
PLoS Pathog (2021) 17 (4), e1009490 DOI: 10.1371/journal.ppat.1009490
Cited by 13
AI Summary: OriGene’s goat-anti-chicken-IgY-FITC secondary antibody was used to detect chicken antibodies in cell-based immunofluorescence assays, enabling visualization of viral antigen-antibody interactions in the study.
Snippet: r 1 hour of incubation we washed the cells three times with washing buffer (0,025% Tween-20/PBS), before adding the fluorescein isothiocyanate (FITC) labeled secondary Ab (goat-anti-chicken-IgY-FITC (OriGene Technologies GmbH, Maryland, USA)), diluted 1:400 in blocking buffer. Following 1-hour incubation, the cells were washed three times with washing buffer and finally 20% glycerol in PBS was add...
Showing 1-1 of 1 papers.
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