Streptavidin Protein

CAT#: RA021T

Streptavidin protein, 1 mg

Specifications

Product Data

• Protein Source: S. avidinii
• Concentration: lot specific
• Purity: Prepared from pure Streptavidin as determined by electrophoresis.
• Conjugation: Rhodamine
• Buffer: State: Lyophilized purifed Ig fraction
  Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons).
  Absorption/Emission Wavelength: 550 nm/570 nm
  FITC/Protein Ratio: 2.7 moles TRITC per mole of Streptavidin.
  Preservative: 0.01% (w/v) Sodium Azide
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Presentation Label: Rhodamine
• Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purifed Ig fraction
• Applications: FLISA: 1/10,000 - 1/50,000.
  Immunofluorescence: 1/1,000-1/5,000.
  Fluorescent Western blotting.
• Protein Description: Rhodamine conjugated Streptavidin
• Note: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Streptavidin.
  No reaction was observed against anti-Avidin.
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: one year from despatch.