Streptavidin Protein

CAT#: RA021PE

Streptavidin protein, 1 ml

Specifications

Product Data

• Protein Source: S. avidinii
• Concentration: lot specific
• Purity: Prepared from electrophoretically purified streptavidin isolated from Streptomyces avidinii conjugated to the chromophore R-Phycoerythrin. Free fluorochrome is removed by tandem molecular sieve chromatographies.
• Conjugation: PE
• Buffer: State: Lyophilized purified Ig fraction
  Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml BSA as a stabilizer and 0.01% Sodium Azide as a preservative
  Label: R-Phycoerythrin (R-PE) (MW 240, 000 daltons).
  Absorption/Emission Wavelength: 488 nm/575 nm
  OD566 nm/OD280 nm: 2.5
  Presentation Label: PE
• Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purified Ig fraction
• Applications: This reagent has been specifically designed for the detection of biotinylated biological probes such as antibodies or cytokines in Flow Cytometry or Immunhistochemisty.
  The maximum amount of reagent to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of Streptavdin RPE. Lesser amounts of reagent may be sufficient for staining.
  As a general guideline dilutions of 1/100-1/250 should be suitable for most applications.
  Recommended Dilutions:
  Flow Cytometry: 1/100-1/250.
  IF Microscopy: 1/100-1/250
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.