Streptavidin Protein

CAT#: RA021AP

Streptavidin protein, 1 mg

Specifications

Product Data

• Protein Source: S. avidinii
• Concentration: lot specific
• Purity: Prepared from Electrophoretically pure Streptavidin.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin. No reaction was observed against anti-Avidin.
• Conjugation: AP
• Buffer: State: Liquid (sterile filtered) purified Ig fraction.
  Buffer System: 50 mM Tris Chloride, 150 mM Sodium Chloride, 1 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
  Label: Alkaline Phosphatase (Calf Intestine; Molecular Weight 140, 000 daltons).
  Preservative: 0.05% (w/v) Sodium Azide
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Presentation Label: AP
• Preparation: Liquid (sterile filtered) purified Ig fraction.
• Applications: ELISA: 1/8,000-1/32,000.
  Western blot: 1/1,000-1/4,000.
  Immunohistochemistry: 1/200-1/1,000.
• Protein Description: Streptavidin Alkaline Phosphatase Conjugated
• Storage: Store vial at 2-8°C before opening.
  DO NOT FREEZE!
  This product is stable at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.