Collagen type V Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Protein Source | Placenta |
| Concentration | 1.0 mg/ml by modified Lowry assay |
| Purity | Human Collagen Type V has been prepared from Human Placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Human Collagen Type V reacts with anti-Collagen Type V. Reaction with Rockland’s anti-Collagen I, II, III, IV or VI is negligible. |
| Buffer | 0.5 M Acetic Acid |
| Preparation | Liquid. |
| Recommended Dilution | ELISA,Immunohistochemistry,Western Blot |
| Protein Description | This product as been prepared from Human Placenta and is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Reacts with anti-Collagen Type V. Reaction with anti-Collagen I, II, III, IV or VI is negligible (typically less than 1% cross reactivity was detected by ELISA). |
| Note | Human Collagen Type V has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
| Storage | Store vial at 4° C prior to opening. Human Collagen Type V is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is six (6) months from date of receipt. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_000084 |
| Locus ID | 1289 |
| Cytogenetics | 9q34.3 |
| Synonyms | Type V collagen, collagen 5, human collagen |
| Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, May 2013 |
| Protein Categories | Intracellular Proteins, Secreated Proteins |
| Protein Families | for Immunoprecipitation and for Western blotting., Suitable for use as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
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