Collagen type III alpha 1 chain Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Protein Source | Placenta |
| Concentration | 1.0 mg/ml by nanodrop at 205 nm |
| Purity | Human Collagen Type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with Anti-Collagen Type III. Reaction with Rockland’s Anti-Collagen I, II, IV, V or VI is negligible. |
| Buffer | 0.5 M Acetic Acid |
| Preparation | Liquid (sterile filtered) |
| Recommended Dilution | ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot |
| Protein Description | This product has been prepared from human placenta and is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Reacts with anti-Collagen Type III. Reaction with anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA). |
| Note | Human Collagen Type III purified protein standard has been tested in SDS-Page and western blot and is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation, and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen Type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure. |
| Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is six (6) months from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_000081 |
| Locus ID | 1281 |
| Cytogenetics | 2q32.2 |
| Synonyms | Type III collagen, collagen 3, human collagen, Alpha 1 type III collagen, Alpha1 (III) collagen, COL3A1_HUMAN, Collagen Type III Alpha 1 Chain, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Fetal Collagen, Human placenta Collagen Type III Protein, Human Type III Collagen Protein, scleroprotein collagen |
| Summary | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. provided by R. Dalgleish, Feb 2008 |
| Protein Categories | Cytokines, Growth Factors, Intracellular Proteins, Secreated Proteins |
| Protein Families | for Immunoprecipitation and for Western blotting., Suitable for use as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
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