Streptavidin Protein
Specifications
| Product Data | |
| Protein Source | S. avidinii |
|---|---|
| Predicted MW | 53 kDa |
| Purity | >95% pure (SDS-PAGE) |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized Streptavidin from Streptomyces avidinii (Essentially salt free) Buffer System: PBS Preservative: 1% Mannitol Stabilizer: 1% Dextran |
| Bioactivity | Biological: One unit will bind 1 µg of D-Biotin. Specific: 17 U/mg Streptavidin |
| Reconstitution Method | Restore with distilled water or saline. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. |
| Preparation | Lyophilized Streptavidin from Streptomyces avidinii (Essentially salt free) |
| Recommended Dilution | Can be used for biotinylated antibody detection and quantification by ELISA, blotting and immunohistology; as a ligand for immobilization on activated matrices. |
| Protein Description | Purified Streptavidin prepared by Affinity Chromatography |
| Note | pI: 5 |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
| Synonyms | StAv |
| Summary | Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. |
| Protein Families | blotting and immunohistology; as a ligand for immobilization on activated matrices., Can be used for biotinylated antibody detection and quantification by ELISA |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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