Streptavidin Protein

SKU BA662

Streptavidin protein, 5 mg

Product Data

• Protein Source: S. avidinii
• Predicted MW: 53 kDa
• Purity: >95% pure (SDS-PAGE)
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Lyophilized Streptavidin from Streptomyces avidinii (Essentially salt free); Buffer System: PBS; Preservative: 1% Mannitol; Stabilizer: 1% Dextran
• Bioactivity: Biological: One unit will bind 1 µg of D-Biotin.; Specific: 17 U/mg Streptavidin
• Reconstitution Method: Restore with distilled water or saline. ; Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
• Preparation: Lyophilized Streptavidin from Streptomyces avidinii (Essentially salt free)
• Recommended Dilution: Can be used for biotinylated antibody detection and quantification by ELISA, blotting and immunohistology; as a ligand for immobilization on activated matrices.
• Protein Description: Purified Streptavidin prepared by Affinity Chromatography
• Note: pI: 5
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient

Reference Data

• Synonyms: StAv
• Summary: Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
• Protein Families: blotting and immunohistology; as a ligand for immobilization on activated matrices., Can be used for biotinylated antibody detection and quantification by ELISA