Filamin Chicken Protein
Specifications
| Product Data | |
| Species | Chicken |
|---|---|
| Protein Source | Gizzard |
| Predicted MW | 250 kDa |
| Concentration | lot specific |
| Purity | >90% > 90% (determined by SDS gelelectrophoresis) |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized Buffer System: 20 mM Tris/acetate buffer pH 7.6, 0.1 mM EDTA, 2 mM DTT, 20 mM NaCl |
| Reconstitution Method | Restore with 250 µl Laemmli buffer (final volume 250 µl) or 9M urea buffer (e.g. IEF or NEPHGE buffer) Sometimes filamin is not readily soluble after lyophilization. In this case add 250 µl dist. water, after dissolution add 135 mg solid urea (to reach an end concentration of 9 M urea), leave for 1h at room temperature, separate insoluble rests by a short centrifugation step. |
| Preparation | Lyophilized |
| Recommended Dilution | Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. |
| Protein Description | Filamin, purified polypeptide |
| Note | Isoelectric Point: pI 6.4 |
| Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store the protein undiluted at -70°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | FLNA, FNLB, FNLC, FN1, FN2, FN3 |
| Protein Families | Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
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