Chicken IgM (Mu chain) Chicken Protein
Specifications
| Product Data | |
| Species | Chicken |
|---|---|
| Protein Source | Serum |
| Concentration | 1.0mg/ml by UV absorbance at 280 nm |
| Purity | Chicken IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Chicken IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and anti-Chicken IgM (µ chain specific). No reaction was observed against anti-Chicken IgG F(c). Some light chain cross reactivity will occur with anti-Chicken IgG. Analysis by SDS-PAGE was used to show purity at greater than 95%. Some high molecular weight banding may be visible. A minor band at ~50 kDa may also be visible. |
| Presentation State | Purified |
| Buffer | 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 |
| Preparation | Liquid |
| Recommended Dilution | ELISA,Immunohistochemistry,Western Blot |
| Protein Description | Chicken IgM |
| Note | Chicken IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
| Storage | Chicken IgM whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | Chicken Immunoglobulin M, Fowl IgM |
| Summary | Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. |
| Protein Families | as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins., Suitable for use as antigen or ligand in immunochemical reactions |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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