Bovine IgG (Fab Fragment) Bovine Protein
Specifications
| Product Data | |
| Species | Bovine |
|---|---|
| Protein Source | Serum |
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Papain. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Preparation | Lyophilized IgG Fab fragment |
| Protein Description | Biotin Conjugated Bovine IgG Fab fragment. |
| Note | Bovine IgG F(ab) fragment biotin conjugation can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | Bovine IgG F(ab) fragment biotin conjugation, BAC conjugated Bovine IgG Fab fragment |
| Summary | Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. |
| Protein Families | Western Blot. ELISA. Immunohistochemistry. Immunofluorescence. |
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