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Bovine IgG (F(ab')2 Fragment) Bovine Protein

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SKU
AR08014HR-N
Bovine IgG F(ab')2 Peroxidase
Size:
1 mg
  $475.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Species Bovine
Protein Source Serum
Concentration 1.0 mg/mL by UV absorbance at 280 nm
Purity This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum.  No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin.
Conjugation HRP
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Preparation Lyophilized
Protein Description Peroxidase Conjugated bovine IgG F(ab)2 fragment.
Note BOVINE IgG F(ab'2) fragment Peroxidase conjugated is designed as a control or standard reagent in Western Blotting and ELISA experiments.
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Reference Data
Synonyms Bovine IgG F(ab')2 Peroxidase conjugation, Bovine IgG F(ab')2 HRP conjugation, Peroxidase conjugated Bovine IgG Fab2, HRP conjugated Bovine IgG Fab2
Summary Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Protein Families Western Blot.
ELISa.
Immunohistochemistry.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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