Bovine IgG (F(ab')2 Fragment) Bovine Protein
Specifications
| Product Data | |
| Species | Bovine |
|---|---|
| Protein Source | Serum |
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin. |
| Conjugation | FITC |
| Buffer | 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Preparation | Lyophilized |
| Protein Description | Fluorescein Conjugated bovine IgG F(ab)2 fragment. |
| Note | BOVINE IgG F(ab')2 fragment Fluorescein conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | Bovine IgG F(ab')2 Fluorescein conjugation, Bovine IgG F(ab')2 FITC conjugation, Fluorescein conjugated Bovine IgG Fab2 fragment, FITC conjugated Bovine IgG Fab2 fragment |
| Summary | Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region. |
| Protein Families | Flow Cytometry. Immunofluorescence. |
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