POLR2J2 (NM_032959) Human Mass Spec Standard

CAT#: PH324755

POLR2J2 MS Standard C13 and N15-labeled recombinant protein (NP_116581)

Product Images

Coomassie blue staining of purified POLR2J2 protein (Cat# TP324755). The protein was produced from HEK293T cells transfected with POLR2J2 cDNA clone (Cat# RC224755) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC224755
• Predicted MW: 12.9 kDa
• Protein Sequence:
  Sequence Link

  >RC224755 representing NM_032959
  Red=Cloning site Green=Tags(s)
  
  MNAPPAFESFLLFEGEKITINKDTKVPNACLFTMNKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKI
  IIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRTCLLPLRLLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_116581
• RefSeq Size: 1727
• RefSeq ORF: 345
• Synonyms: HRPB11B; POLR2J3; RPB11b1; RPB11b2
• Locus ID: 246721
• UniProt ID: Q9GZM3
• Cytogenetics: 7q22.1
• Summary: This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase